Kunz H W, Cortese Hassett A L, Inomata T, Misra D N, Gill T J
Department of Pathology, University of Pittsburgh School of Medicine, PA 15261.
Immunogenetics. 1989;30(3):181-7. doi: 10.1007/BF02421204.
A new antigenic system in the rat homologous to the Qa/TL antigen system in the mouse has been characterized. It was detected by antibodies raised in donor-recipient combinations that were matched for the RT1.A, B, D, E loci in the major histocompatibility complex (MHC): (R11 X BN)F1 anti-BN.1L(LEW), (R18 X BN)F1 anti-BN.1L, and BN.1LV1(F344) anti-BN.1L. Absorption analyses using these antisera and a variety of inbred, congenic, and recombinant strains identified three alleles, RT1.Ga, Gb, Gc, of which Gc is a null allele. The strain distribution of these alleles was determined using 37 strains of rats representative of all of the prototypic haplotypes and a number of congenic and recombinant strains. The use of the congenic and recombinant strains showed that the RT1.G locus was linked to the MHC and that the most probable gene order was A-E-G. Testcross analysis showed that the map distance between A and G was 1.4 cM (4/285 recombinants). The RT1.G antigen has a heavy chain of Mr 46,000 and is present on both T and B cells.
已对大鼠中与小鼠的Qa/TL抗原系统同源的一种新抗原系统进行了表征。它是通过在主要组织相容性复合体（MHC）中RT1.A、B、D、E位点相匹配的供体-受体组合中产生的抗体检测到的：（R11×BN）F1抗BN.1L（LEW）、（R18×BN）F1抗BN.1L以及BN.1LV1（F344）抗BN.1L。使用这些抗血清以及多种近交系、同源近交系和重组品系进行的吸收分析确定了三个等位基因，即RT1.Ga、Gb、Gc，其中Gc为无效等位基因。使用代表所有原型单倍型的37种大鼠品系以及一些同源近交系和重组品系确定了这些等位基因的品系分布。同源近交系和重组品系的使用表明，RT1.G位点与MHC连锁，最可能的基因顺序为A-E-G。测交分析表明，A与G之间的图距为1.4 cM（4/285个重组体）。RT1.G抗原具有一条46,000道尔顿的重链，存在于T细胞和B细胞上。
