Ayene S I, Srivastava P N
Radiation Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Int J Radiat Biol. 1989 Sep;56(3):265-75. doi: 10.1080/09553008914551431.
The effect of WR-2721 on radiation-induced lipid peroxidation and enzyme release was studied after gamma-irradiation of microsomes and erythrocytes. WR-2721 rendered protection to microsomes by reducing lipid peroxidation whereas it did not exert significant protection against radiation-induced lipid peroxidation in erythrocytes. The non-protective effect of WR-2721 was also observed in radiation-induced enzyme (AChE) release of erythrocytes. It is proposed that erythrocytes do not dephosphorylate WR-2721 into the free thiol derivative (WR-1065). This was confirmed by the microsomal treatment of erythrocytes that had shown the protective effect of WR-2721. Further, a significant increase in sulphydryl content in microsomes, and no such increase in erythrocytes, after the WR-2721 treatment indicated that dephosphorylation of WR-2721 is necessary for its protective effect.
在微粒体和红细胞经γ射线照射后，研究了WR-2721对辐射诱导的脂质过氧化和酶释放的影响。WR-2721通过减少脂质过氧化对微粒体起到保护作用，而对红细胞辐射诱导的脂质过氧化未发挥显著保护作用。在红细胞辐射诱导的酶（乙酰胆碱酯酶）释放中也观察到WR-2721的非保护作用。有人提出，红细胞不会将WR-2721去磷酸化为游离硫醇衍生物（WR-1065）。经微粒体处理的红细胞显示出WR-2721的保护作用，这证实了上述观点。此外，WR-2721处理后微粒体中巯基含量显著增加，而红细胞中未出现这种增加，这表明WR-2721的去磷酸化对其保护作用是必要的。
