Lupatov A Yu, Vdovin A S, Vakhrushev I V, Poltavtseva R A, Yarygin K N
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia,
Bull Exp Biol Med. 2015 Feb;158(4):537-43. doi: 10.1007/s10517-015-2803-2. Epub 2015 Feb 24.
Expression of 20 surface markers was analyzed in cultures of mesenchymal stromal cells of the umbilical cord, fibroblasts from adult and fetal human skin, and fibroblast-like cells of fetal liver was analyzed by fl ow cytometry. The studied cultures did not express hemopoietic cells markers, but were positive for CD73, CD90, and CD105 markers recommended by the International Society of Cell Therapy for the identification of the multipotent mesenchymal stromal cells. Fetal liver fibroblast-like cells were positive for CD54; this marker was absent in skin fibroblast cultures, but was expressed by umbilical cord mesenchymal stromal cells. Further study of these cells revealed a minor subpopulation of cells co-expressing CD24 and CD90 or CD24 and CD54. We hypothesized that these cells probably participate in epithelial mesenchymal transition.
通过流式细胞术分析了脐带间充质基质细胞、成人及胎儿人皮肤成纤维细胞和胎儿肝成纤维样细胞培养物中20种表面标志物的表达情况。所研究的培养物不表达造血细胞标志物，但对国际细胞治疗协会推荐用于鉴定多能间充质基质细胞的CD73、CD90和CD105标志物呈阳性。胎儿肝成纤维样细胞对CD54呈阳性；该标志物在皮肤成纤维细胞培养物中不存在，但脐带间充质基质细胞表达。对这些细胞的进一步研究发现了一个共表达CD24和CD90或CD24和CD54的细胞亚群。我们推测这些细胞可能参与上皮-间充质转化。
