Laboratório de Biologia Molecular e Genômica, Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário s/n, Lagoa Nova, Natal, RN 59072-970, Brazil.
Regen Med. 2012 Mar;7(2):147-57. doi: 10.2217/rme.11.113.
To conduct a morphological, functional and chromosomal characterization of mesenchymal stem cell populations from the human subendothelium umbilical cord vein after cryopreservation.
MATERIAL & METHODS: Five human umbilical cords were processed in order to obtain mesenchymal stem cells. Flow cytometry, differentiation assays and cytogenetic analysis were carried out before and after the cryopreservation process.
Flow cytometry revealed that CD105, CD73 and CD90 markers were expressed by the cells, which lacked the expression of hematopoietic lineage markers, such as CD14, CD34 and CD45. The mesenchymal stem cells demonstrated capacity for osteogenic, adipogenic and chondrogenic differentiation. Chromosome analysis showed no clonal chromosome changes in the cells in either situation. However, a significant number of nonclonal chromosomal aberrations were apparent after cryopreservation, including monosomies and structural changes. Cells isolated from one umbilical cord exhibited a rare balanced paracentric inversion, likely a cytogenetic constitutional alteration. This was present both before and after experimental procedures.
These findings show that using mesenchymal stem cells for clinical approaches requires careful investigation and sensitive tests in order to ensure cellular therapy biosafety.
对冷冻保存后的人脐静脉内皮下间质干细胞群体进行形态学、功能和染色体特征分析。
为了获得间充质干细胞，对 5 个人脐带进行了处理。在冷冻保存前后进行了流式细胞术、分化试验和细胞遗传学分析。
流式细胞术显示，细胞表达 CD105、CD73 和 CD90 标志物，缺乏造血谱系标志物的表达，如 CD14、CD34 和 CD45。间充质干细胞具有成骨、成脂和成软骨分化的能力。染色体分析显示，在两种情况下细胞均无克隆染色体改变。然而，冷冻保存后明显出现了大量非克隆染色体异常，包括单体和结构改变。从一条脐带中分离出的细胞表现出罕见的平衡旁中心倒位，可能是细胞遗传学的结构改变。这在实验前后都存在。
这些发现表明，使用间充质干细胞进行临床方法需要仔细的研究和敏感的测试，以确保细胞治疗的生物安全性。
