Martins Frederico Severino, Pascoa Henrique, de Paula José Realino, da Conceição Edemilson Cardoso
Department of Pharmacy, Faculty of Pharmacy Research, Federal University of Goiás, Goiás, Brazil.
Pharmacogn Mag. 2015 Jan-Mar;11(41):226-31. doi: 10.4103/0973-1296.149742.
Despite the increased use of Brosimum gaudichaudii roots as raw material on medicine to treatment of vitiligo, there are not studies that showing the impact of unit operations on the quality and standardized of the extract of B. gaudichaudii. The quality of the herbal extract is essential to ensure the safety and efficacy of pharmaceutical product. Due the medical and commercial importance, this study aimed to evaluate the impact of the extraction method (ultrasound or percolation) on the quality of herbal extract and optimize the extraction of psoralen and 8-methoxypsoralen (8-MOP) from B. gaudichaudii.
The extraction recovery was evaluate by high-performance liquid chromatography (C8 reverse phase column and acetonitrile: Water 45:55 and flow rate 0.6 mL/min). The extraction was performed by ultrasound-assisted extraction (UEA) or percolation using a Box-Behnken design.
From both chemical markers (psoralen and bergapten), the optimal conditions for the UEA were an extraction time of 25 min, the mean particle size of 100 μm, and an ethanol: Water ratio of 55:45 (v/v).
The extraction by percolation revealed that ethanol 55% was more efficient than ethanol 80% to extract psoralen and bergapten.
尽管圭亚那牛乳树（Brosimum gaudichaudii）根作为治疗白癜风药物的原材料使用越来越多，但尚无研究表明单元操作对圭亚那牛乳树提取物的质量和标准化的影响。草药提取物的质量对于确保药品的安全性和有效性至关重要。鉴于其医学和商业重要性，本研究旨在评估提取方法（超声或渗漉）对草药提取物质量的影响，并优化从圭亚那牛乳树中提取补骨脂素和8-甲氧基补骨脂素（8-MOP）的工艺。
采用高效液相色谱法（C8反相柱，乙腈：水45:55，流速0.6 mL/min）评估提取回收率。使用Box-Behnken设计通过超声辅助提取（UEA）或渗漉进行提取。
从两种化学标志物（补骨脂素和佛手柑内酯）来看，超声辅助提取的最佳条件为提取时间25分钟、平均粒径100μm、乙醇：水比例55:45（v/v）。
渗漉提取表明，55%乙醇比80%乙醇提取补骨脂素和佛手柑内酯的效率更高。
