Liu Na, Tang Zhouping, Yu Zhiyuan, Xie Minjie, Zhang Yu, Yang Erfang, Xu Shabei
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
Neural Regen Res. 2012 Jun 5;7(16):1213-9. doi: 10.3969/j.issn.1673-5374.2012.16.002.
This study aimed to examine the differences in the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds and in two-dimensional culture on common flat culture plates. The proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture, as detected by an MTT assay. In addition, more than half of the olfactory ensheathing cells subcultured using the trypsinization method in three-dimensional culture displayed a spindly Schwann cell-like morphology with extremely long processes, while they showed a flat astrocyte-like morphology in two-dimensional culture. Moreover, spindle-shaped olfactory ensheathing cells tended to adopt an elongated bipolar morphology under both culture conditions. Experimental findings indicate that the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds are better than those in two-dimensional culture.
本研究旨在探讨嗅鞘细胞在硫酸乙酰肝素胶原生物支架上进行三维培养以及在普通平面培养板上进行二维培养时，其形态学特性和增殖情况的差异。通过MTT法检测发现，嗅鞘细胞在三维培养中的增殖率高于二维培养。此外，在三维培养中采用胰蛋白酶消化法传代培养的嗅鞘细胞，超过半数呈现出具有极长突起的梭形雪旺细胞样形态，而在二维培养中则呈现扁平的星形胶质细胞样形态。而且，在两种培养条件下，纺锤形的嗅鞘细胞都倾向于呈现细长的双极形态。实验结果表明，嗅鞘细胞在硫酸乙酰肝素胶原生物支架上进行三维培养时的形态学特性和增殖情况优于二维培养。
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