Wang Zong-Jie, Liu Qian-Qian, Zhao Li-Hua, Du Zong-Jun, Chen Guan-Jun
College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2015 May;65(Pt 5):1542-1549. doi: 10.1099/ijs.0.000135. Epub 2015 Feb 23.
A novel Gram-stain-negative, rod-shaped, gliding, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated FA350(T), was isolated from coastal sediment from Xiaoshi Island, Weihai, China. Strain FA350(T) showed growth on modified nutrient agar supplemented with 0.1% d-(+)-trehalose and with distilled water replaced by seawater. Optimal growth occurred at 33 °C and pH 8.5 with 4% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FA350(T) belongs to a novel bacterial order in the class Deltaproteobacteria , and the most closely related type strains belong to the order Desulfuromonadales , with 85.1-85.6% 16S rRNA gene sequence similarity. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol and two unknown phospholipids. Major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 and iso-C17 : 1ω10c and menaquinone MK-7 was the sole respiratory quinone. The DNA G+C content of strain FA350(T) was 60.3 mol%. The isolate and closely related environmental clones formed a novel order-level clade in the class Deltaproteobacteria . Comparative analysis of 16S rRNA gene sequences and characterization indicated that strain FA350(T) may represent a novel order of the Deltaproteobacteria . Here, we propose the name Bradymonas sediminis gen. nov., sp. nov. to accommodate strain FA350(T). The type strain of Bradymonas sediminis is FA350(T) ( =DSM 28820(T) =CICC 10904(T)); Bradymonadales ord. nov. and Bradymonadaceae fam. nov. are also proposed to accommodate the novel taxon.
从中国威海小石岛的海岸沉积物中分离出一株新型革兰氏阴性、杆状、滑行、兼性厌氧、氧化酶阴性且过氧化氢酶阳性的细菌，命名为FA350(T)。菌株FA350(T)在添加0.1% d-(+)-海藻糖且用海水代替蒸馏水的改良营养琼脂上生长。在33°C、pH 8.5和4% NaCl条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株FA350(T)属于δ-变形菌纲中的一个新细菌目，与其关系最密切的模式菌株属于脱硫单胞菌目，16S rRNA基因序列相似性为85.1-85.6%。该新菌株的极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油和两种未知磷脂组成。主要细胞脂肪酸为异-C15 : 0、异-C17 : 0和异-C17 : 1ω10c，甲基萘醌MK-7是唯一的呼吸醌。菌株FA350(T)的DNA G+C含量为60.3 mol%。该分离株及密切相关的环境克隆在δ-变形菌纲中形成了一个新的目级分支。16S rRNA基因序列的比较分析和特征表明，菌株FA350(T)可能代表δ-变形菌纲中的一个新目。在此，我们提议将其命名为迟缓单胞菌属（Bradymonas），新属，新种，以容纳菌株FA350(T)。迟缓单胞菌的模式菌株为FA350(T)（=DSM 28820(T)=CICC 10904(T)）；还提议设立迟缓单胞菌目（Bradymonadales），新目和迟缓单胞菌科（Bradymonadaceae），新科来容纳这个新分类单元。
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