UDP-葡萄糖基转移酶71c5是一种主要的葡萄糖基转移酶，在拟南芥中介导脱落酸的体内平衡。
UDP-glucosyltransferase71c5, a major glucosyltransferase, mediates abscisic acid homeostasis in Arabidopsis.
作者信息
Liu Zhen, Yan Jin-Ping, Li De-Kuan, Luo Qin, Yan Qiujie, Liu Zhi-Bin, Ye Li-Ming, Wang Jian-Mei, Li Xu-Feng, Yang Yi
机构信息
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.).
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Sichuan 610064, China (Z.L., J.-P.Y., D.-K.L., Q.L., Q.Y., Z.-B.L., J.-M.W., X.-F.L., Y.Y.);State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Sichuan 610065, China (Z.L., D.-K.L., Y.Y.);Biotechnology Research Center of Life Science and Technology College, Kunming University of Science and Technology, Yunnan 650500, China (J.P.-Y.); and West China School of Pharmacy, Sichuan University, Sichuan 610041, China (L.-M.Y.)
出版信息
Plant Physiol. 2015 Apr;167(4):1659-70. doi: 10.1104/pp.15.00053. Epub 2015 Feb 20.
Abscisic acid (ABA) plays a key role in plant growth and development. The effect of ABA in plants mainly depends on its concentration, which is determined by a balance between biosynthesis and catabolism of ABA. In this study, we characterize a unique UDP-glucosyltransferase (UGT), UGT71C5, which plays an important role in ABA homeostasis by glucosylating ABA to abscisic acid -: glucose ester (GE) in Arabidopsis (Arabidopsis thaliana). Biochemical analyses show that UGT71C5 glucosylates ABA in vitro and in vivo. Mutation of UGT71C5 and down-expression of UGT71C5 in Arabidopsis cause delay in seed germination and enhanced drought tolerance. In contrast, overexpression of UGT71C5 accelerates seed germination and reduces drought tolerance. Determination of the content of ABA and ABA-GE in Arabidopsis revealed that mutation in UGT71C5 and down-expression of UGT71C5 resulted in increased level of ABA and reduced level of ABA-GE, whereas overexpression of UGT71C5 resulted in reduced level of ABA and increased level of ABA-GE. Furthermore, altered levels of ABA in plants lead to changes in transcript abundance of ABA-responsive genes, correlating with the concentration of ABA regulated by UGT71C5 in Arabidopsis. Our work shows that UGT71C5 plays a major role in ABA glucosylation for ABA homeostasis.
脱落酸（ABA）在植物生长发育过程中起着关键作用。ABA在植物中的作用主要取决于其浓度，而ABA的浓度由其生物合成与分解代谢之间的平衡所决定。在本研究中，我们鉴定了一种独特的尿苷二磷酸葡萄糖基转移酶（UGT），即UGT71C5，它通过将ABA糖基化为脱落酸葡萄糖酯（GE）在拟南芥（Arabidopsis thaliana）的ABA稳态中发挥重要作用。生化分析表明，UGT71C5在体外和体内均可使ABA糖基化。UGT71C5突变以及UGT71C5在拟南芥中的表达下调会导致种子萌发延迟和耐旱性增强。相反，UGT71C5的过表达会加速种子萌发并降低耐旱性。对拟南芥中ABA和ABA-GE含量的测定表明，UGT71C5突变和UGT71C5表达下调导致ABA水平升高和ABA-GE水平降低，而UGT71C5过表达则导致ABA水平降低和ABA-GE水平升高。此外，植物中ABA水平的改变会导致ABA响应基因转录本丰度的变化，这与拟南芥中由UGT71C5调节的ABA浓度相关。我们的研究表明，UGT71C5在ABA糖基化以维持ABA稳态中起主要作用。
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