可调节淀粉样蛋白形成肽组装成纳米片作为逆转录病毒载体。
Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier.
作者信息
Dai Bin, Li Dan, Xi Wenhui, Luo Fang, Zhang Xiang, Zou Man, Cao Mi, Hu Jun, Wang Wenyuan, Wei Guanghong, Zhang Yi, Liu Cong
机构信息
Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
State Key Laboratory of Surface Physics, Key Laboratory for Computational Physical Sciences (Ministry of Education), Department of Physics, Fudan University, Shanghai 200433, China;
出版信息
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):2996-3001. doi: 10.1073/pnas.1416690112. Epub 2015 Feb 23.
Using and engineering amyloid as nanomaterials are blossoming trends in bionanotechnology. Here, we show our discovery of an amyloid structure, termed "amyloid-like nanosheet," formed by a key amyloid-forming segment of Alzheimer's Aβ. Combining multiple biophysical and computational approaches, we proposed a structural model for the nanosheet that is formed by stacking the amyloid fibril spines perpendicular to the fibril axis. We further used the nanosheet for laboratorial retroviral transduction enhancement and directly visualized the presence of virus on the nanosheet surface by electron microscopy. Furthermore, based on our structural model, we designed nanosheet-forming peptides with different functionalities, elucidating the potential of rational design for amyloid-based materials with novel architecture and function.
将淀粉样蛋白用作纳米材料并对其进行工程化处理是生物纳米技术中蓬勃发展的趋势。在此，我们展示了我们对一种淀粉样蛋白结构的发现，该结构被称为“类淀粉样纳米片”，由阿尔茨海默病Aβ的关键淀粉样蛋白形成片段构成。结合多种生物物理和计算方法，我们提出了一种纳米片的结构模型，该模型是通过将淀粉样纤维的棘突垂直于纤维轴堆叠而成。我们进一步将该纳米片用于实验室逆转录病毒转导增强，并通过电子显微镜直接观察到病毒在纳米片表面的存在。此外，基于我们的结构模型，我们设计了具有不同功能的纳米片形成肽，阐明了对具有新颖结构和功能的基于淀粉样蛋白的材料进行合理设计的潜力。
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