Fox R I, Saito I, Chan E K, Josephs S, Salahuddin S Z, Ahlashi D V, Staal F W, Gallo R, Pei-Ping H, Le C S
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
J Autoimmun. 1989 Aug;2(4):449-55. doi: 10.1016/0896-8411(89)90174-1.
The recent isolation of a new member of the herpes virus family (Human Herpes Virus-6, HHV-6) from patients with lymphoproliferative diseases prompted us to examine biopsies from six patients with primary Sjögren's Syndrome (SS) who developed non-Hodgkin's lymphoma. Five SS patients developed B-cell lymphoma and one developed a T-cell lymphoma based on immunoglobulin and T-cell antigen receptor (TCAR) gene rearrangements. In two SS patients with B-cell lymphomas, viral DNAs were detected, including: (a) Epstein-Barr Virus (EBV) DNA that exhibited an unusual pattern of restriction fragment length polymorphisms (RFLP) of the Bam M viral DNA segment; and (b) HHV-6 DNA in a second SS patient's lymphoma, with an RFLP similar to recent viral isolates from patients with other lymphoproliferative diseases. Viral DNA was not detected in the other four SS lymphoma biopsies. Also, all six biopsies were examined for presence of other viral DNAs (including CMV, HTLV, HIV and adenovirus) and were negative. Antibody titers to EBV-associated early-diffuse-antigen (EA-D), as assessed by ELISA method, and antibody titers against HHV-6, as detected by immunofluorescence and radio-immunoprecipitation assays, were markedly elevated in several SS patients with lymphoma and pseudolymphoma. These results suggest a potential role of EBV or HHV-6 in the neoplastic transformation that occurs with increased frequency in SS patients.
最近从淋巴增生性疾病患者中分离出一种新的疱疹病毒家族成员（人类疱疹病毒6型，HHV - 6），这促使我们对6例原发性干燥综合征（SS）并发非霍奇金淋巴瘤患者的活检组织进行检查。根据免疫球蛋白和T细胞抗原受体（TCAR）基因重排，5例SS患者发生了B细胞淋巴瘤，1例发生了T细胞淋巴瘤。在2例患有B细胞淋巴瘤的SS患者中，检测到病毒DNA，包括：（a）爱泼斯坦 - 巴尔病毒（EBV）DNA，其Bam M病毒DNA片段呈现出异常的限制性片段长度多态性（RFLP）模式；（b）第二例SS患者淋巴瘤中的HHV - 6 DNA，其RFLP与最近从其他淋巴增生性疾病患者中分离出的病毒相似。在其他4例SS淋巴瘤活检组织中未检测到病毒DNA。此外，对所有6份活检组织检测了其他病毒DNA（包括巨细胞病毒、人类嗜T淋巴细胞病毒、人类免疫缺陷病毒和腺病毒），结果均为阴性。通过ELISA法评估，部分患有淋巴瘤和假性淋巴瘤的SS患者中，针对EBV相关早期弥漫性抗原（EA - D）的抗体滴度以及通过免疫荧光和放射免疫沉淀试验检测到的针对HHV - 6的抗体滴度显著升高。这些结果表明EBV或HHV - 6在SS患者中发生率增加的肿瘤转化过程中可能发挥作用。
