College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.
Immune Netw. 2015 Feb;15(1):27-36. doi: 10.4110/in.2015.15.1.27. Epub 2015 Feb 17.
In the present study, we investigated the protection conferred by a live attenuated Salmonella enterica serovar Typhimurium (ST) strain against Salmonella Typhimurium, Salmonella Gallinarum (SG), and Salmonella Enteritidis (SE) infection in layer chickens. Birds were orally primed with the attenuated ST strain at 7 days of age and then boosted at 4 weeks post prime immunization (PPI). Sequential monitoring of plasma IgG and mucosal secretory IgA (sIgA) levels revealed that inoculation with ST induced a significant antibody response to antigens against ST, SE, and SG. Moreover, significant lymphoproliferative responses to the 3 Salmonella serovars were observed in the immunized group. We also investigated protection against virulent ST, SE, and SG strain challenge. Upon virulent SG challenge, the immunized group showed significantly reduced mortality compared to the non-immunized group. The reduced persistence of the virulent ST and SE challenge strains in the liver, spleen, and cecal tissues of the immunized group suggests that immunization with the attenuated ST strain may not only protect against ST infection but can also confer cross protection against SE and SG infection.
在本研究中，我们研究了减毒鼠伤寒沙门氏菌（ST）活菌株对蛋鸡感染鼠伤寒沙门氏菌、鸡伤寒沙门氏菌（SG）和肠炎沙门氏菌（SE）的保护作用。鸡在 7 日龄时口服接种减毒 ST 株，然后在初次免疫后 4 周加强免疫。连续监测血浆 IgG 和黏膜分泌型 IgA（sIgA）水平表明，接种 ST 诱导了针对 ST、SE 和 SG 的抗原的显著抗体反应。此外，在免疫组中观察到针对 3 种沙门氏菌血清型的显著淋巴细胞增殖反应。我们还研究了对强毒 ST、SE 和 SG 株攻毒的保护作用。在强毒 SG 攻毒后，免疫组的死亡率明显低于非免疫组。免疫组中强毒 ST 和 SE 攻毒菌株在肝脏、脾脏和盲肠组织中的持续存在减少表明，用减毒 ST 株免疫不仅可以预防 ST 感染，还可以提供针对 SE 和 SG 感染的交叉保护。
