Akter Taslima, Nooruzzaman Mohammed, Belal Sheikh Mohammad Shariful Hoque, Ahammed Mustak, Uddin Abm Jalal, Parvin Rokshana, Khan Md Abu Hadi Noor Ali, Islam Md Ariful, Hossain Md Mokbul
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
J Adv Vet Anim Res. 2022 Jun 28;9(2):301-309. doi: 10.5455/javar.2022.i597. eCollection 2022 Jun.
Here, we developed and tested the efficacy of a vaccination protocol based on a commercially available live attenuated serovar Gallinarum () in layer chickens.
50 layer chickens of 16 weeks age were obtained and divided into two groups ( = 25), control and vaccinated. The vaccinated group received vaccine at 0.2 ml/bird, s/c route at 16, 18, 30, and 42 weeks of age. At 21 weeks of age, birds from both groups were challenged with orally at 4 × 10 colony-forming unit per bird.
Both rapid serum plate agglutination and enzyme-linked immunosorbent assay demonstrated a rising rate of seroconversion in vaccinated birds across the study period, with a 4% positive rate at 18 weeks, 56% at 21 weeks, 60% at 30 weeks, and 64% at each time point of 42 and 54 weeks. The vaccine showed 100% clinical protection and reduced the shedding in the feces and eggs of the challenged birds. On the contrary, the unvaccinated challenged birds showed clinical signs and lesions typical of infections with morbidity and mortality rates of 36% and 20%, respectively, and had high rates of shedding in feces and eggs.
With the proposed vaccination schedule shedding was prevented, and a high seroconversion was confirmed. To prevent infections in laying flocks, a 3-month interval immunization program is advised starting at the pre-laying stage.
在此，我们研发并测试了一种基于市售减毒活鸡白痢沙门氏菌血清型（）的疫苗接种方案对蛋鸡的有效性。
获取50只16周龄的蛋鸡，分为两组（每组 = 25只），即对照组和接种组。接种组在16、18、30和42周龄时，以每只鸡0.2毫升的剂量通过皮下途径接种疫苗。在21周龄时，两组鸡均经口用每只鸡4×10个菌落形成单位的[病原体名称]进行攻毒。
快速血清平板凝集试验和酶联免疫吸附测定均表明，在整个研究期间，接种疫苗的鸡的血清转化率呈上升趋势，18周龄时阳性率为4%，21周龄时为56%，30周龄时为60%，42周龄和54周龄的每个时间点均为64%。该疫苗显示出100%的临床保护作用，并减少了攻毒鸡粪便和鸡蛋中的[病原体名称]排出。相反，未接种疫苗的攻毒鸡表现出[病原体名称]感染的典型临床症状和病变，发病率和死亡率分别为36%和20%，粪便和鸡蛋中的[病原体名称]排出率很高。
按照建议的疫苗接种程序可预防[病原体名称]排出，并确认有高血清转化率。为预防蛋鸡群中的[病原体名称]感染，建议从产蛋前期开始实施为期3个月的间隔免疫程序。
