Leung Elvis M K, Chan Wan
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
Chem Res Toxicol. 2015 Apr 20;28(4):567-9. doi: 10.1021/acs.chemrestox.5b00021. Epub 2015 Feb 27.
Balkan endemic nephropathy (BEN) is a peculiar renal disease affecting thousands of farmers living in the suburban areas of the Balkan countries. Emerging evidence suggested that BEN is an environmental disease caused by chronic food poisoning with aristolochic acid (AA). We have developed a sensitive liquid chromatography-tandem mass spectrometry method to detect urinary RNA-AA adducts. Results revealed high levels of RNA-AA adducts in the urine samples collected from AA-treated rats. To the best of our knowledge, this study is the first to report on the detection of urinary RNA-AA adducts. Compared with previous studies that quantified DNA-AA adducts, this method is more sensitive and user-friendly.
巴尔干地方性肾病（BEN）是一种特殊的肾脏疾病，影响着生活在巴尔干国家郊区的数千名农民。新出现的证据表明，BEN是一种由马兜铃酸（AA）慢性食物中毒引起的环境疾病。我们开发了一种灵敏的液相色谱 - 串联质谱法来检测尿中RNA - AA加合物。结果显示，从经AA处理的大鼠收集的尿液样本中RNA - AA加合物水平很高。据我们所知，本研究首次报道了尿中RNA - AA加合物的检测。与之前定量DNA - AA加合物的研究相比，该方法更灵敏且更易于使用。
