抗P-糖蛋白单克隆抗体对无胸腺小鼠体内多药耐药性人类肿瘤生长的抑制作用
Inhibition of multidrug-resistant human tumor growth in athymic mice by anti-P-glycoprotein monoclonal antibodies.
作者信息
Tsuruo T, Hamada H, Sato S, Heike Y
机构信息
Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo.
出版信息
Jpn J Cancer Res. 1989 Jul;80(7):627-31. doi: 10.1111/j.1349-7006.1989.tb01688.x.
Abstract
In an effort to devise an effective treatment for human drug-resistant cancers, we have developed monoclonal antibodies, MRK16 and 17, reactive to the multidrug transporter protein, P-glycoprotein. The monoclonal antibodies given intravenously effectively prevented tumor development in athymic mice inoculated subcutaneously with drug-resistant human ovarian cancer cells 2780AD. Treatment with MRK16 induced rapid regression of established subcutaneous tumors and apparent cures of some animals. Complement-dependent cytotoxicity (MRK16) and antibody-dependent cell-mediated cytolysis (MRK16 and 17) were observed with these antibodies. These monoclonal antibodies may have potential as treatment tools against multidrug resistant human tumors possessing the P-glycoprotein.
摘要
为了设计出一种有效的人类耐药性癌症治疗方法，我们开发了单克隆抗体MRK16和17，它们可与多药转运蛋白P-糖蛋白发生反应。静脉注射这些单克隆抗体可有效预防在无胸腺小鼠皮下接种耐药性人类卵巢癌细胞2780AD后肿瘤的发展。用MRK16治疗可使已形成的皮下肿瘤迅速消退，并且部分动物明显治愈。观察到这些抗体具有补体依赖性细胞毒性（MRK16）和抗体依赖性细胞介导的细胞溶解作用（MRK16和17）。这些单克隆抗体可能具有作为针对具有P-糖蛋白的多药耐药性人类肿瘤的治疗工具的潜力。
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