Herlyn D, Koprowski H
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4761-5. doi: 10.1073/pnas.79.15.4761.
Monoclonal antibodies of IgG2a isotype specifically inhibited growth of human tumors in nude mice. Twenty-three monoclonal antibodies of other isotypes showed no tumoricidal reactivity. Complement depletion of nude mice had no effect on tumor suppression by monoclonal antibody. The role of T and killer cells as mediators of the monoclonal antibody effect in nude mice was virtually excluded. On the other hand, macrophages were strongly incriminated as effector cells because silica treatment of nude mice abolished the tumoricidal effect of monoclonal antibody. IgG2a monoclonal antibody-dependent macrophage-mediated cytotoxicity assays with human tumor cells in culture resulted in specific lysis of tumor cells.
IgG2a 同种型的单克隆抗体可特异性抑制裸鼠体内人肿瘤的生长。其他同种型的 23 种单克隆抗体未显示出杀瘤反应性。裸鼠的补体耗竭对单克隆抗体的肿瘤抑制作用没有影响。T 细胞和杀伤细胞作为裸鼠中单克隆抗体效应介质的作用实际上被排除。另一方面，巨噬细胞被强烈认为是效应细胞，因为对裸鼠进行二氧化硅处理消除了单克隆抗体的杀瘤作用。在培养中用人肿瘤细胞进行的 IgG2a 单克隆抗体依赖性巨噬细胞介导的细胞毒性试验导致肿瘤细胞的特异性裂解。
