Department of Plant Science, Waite Institute, University of Adelaide, 5064, Glen Osmond, S.A., Australia.
Theor Appl Genet. 1996 May;92(7):858-64. doi: 10.1007/BF00221898.
Barley yellow dwarf luteovirus (BYDV) causes serious yield losses in all cereals worldwide. The Yd2 gene from a number of Ethiopian barleys (Hordeum vulgare L.) has been the most effective means of providing resistance against BYDV in cultivated barley. Isolation of the Yd2 gene will enable characterisation of the molecular basis of the Yd2-BYDV interaction. This paper describes the first stage in a project to isolate the gene: the construction of a detailed linkage map of the Yd2 region. The map encompasses 27.6 centiMorgans (cM) of chromosome 3 and contains 19 RFLPs, 2 morphological marker loci, the centromere and Yd2. In the mapping population of 106 F2 individuals, Yd2 perfectly cosegregated with the RFLP loci Xwg889 and XYlp, which were located on the long arm, 0.5 cM from the centromere. The two morphological marker loci, uzu dwarfand white stripe j, both mapped distal to Yd2. The protein product of the gene at the XYlp locus will provide a convenient assay for the selection of Yd2 during the breeding of BYDV-resistant barley varieties.
大麦黄花叶病毒（BYDV）在全球范围内对所有谷物造成严重的产量损失。来自埃塞俄比亚大麦（Hordeum vulgare L.）的 Yd2 基因是在栽培大麦中提供抗 BYDV 最有效的手段。分离 Yd2 基因将使 Yd2-BYDV 相互作用的分子基础得以阐明。本文描述了一个分离该基因的项目的第一阶段：Yd2 区域的详细连锁图谱的构建。该图谱涵盖了 3 号染色体的 27.6 厘摩（cM），包含 19 个 RFLP、2 个形态标记基因座、着丝粒和 Yd2。在 106 个 F2 个体的作图群体中，Yd2 与位于染色体臂上的 RFLP 标记 Xwg889 和 XYlp 完全连锁，距着丝粒 0.5 cM。两个形态标记基因座 uzu 矮化和白条 j 均位于 Yd2 的远端。位于 XYlp 基因座的基因的蛋白质产物将为在培育抗 BYDV 的大麦品种时选择 Yd2 提供一种方便的检测方法。
