Neuss Sabine, Panfil Claudia, Duarte Campos Daniela Filipa, Weber Michael, Otten Christian, Reisgen Uwe, Fischer Horst
Biomed Tech (Berl). 2015 Jun;60(3):215-23. doi: 10.1515/bmt-2014-0106.
Several studies focusing on bone tissue engineering demonstrated that given microstructuring of an implant surface has a strong effect on its interaction with cells, and their adhesion and differentiation. In the present study, geometrically structured titanium alloy surfaces are shown to be able to guide cell adhesion during differentiation in vitro. For this reason, using an electron beam texturing technique, TiAl6V4 surfaces were selectively targeted in the micrometer range. The effect of such textured titanium alloy surfaces on cell adhesion during osteogenic differentiation was analyzed for human mesenchymal stem cells (MSC), the natural precursor cells of bone tissue. Cytotoxicity, cell viability and differentiation were analyzed. Immunofluorescence stainings demonstrated that in contrast to MSC in an expansion medium, MSC in an osteogenic induction medium produce adhesion proteins such as ß3-integrins and thereby connect in an oriented way to the generated microstructures on titanium alloy surfaces. These results are of relevance for developing tailored titanium alloy implant surfaces which exhibit an improved cell response.
多项专注于骨组织工程的研究表明，植入物表面特定的微观结构对其与细胞的相互作用及其黏附与分化有很强的影响。在本研究中，几何结构的钛合金表面被证明能够在体外分化过程中引导细胞黏附。因此，利用电子束纹理技术，在微米范围内对TiAl6V4表面进行了选择性处理。针对骨组织的天然前体细胞——人间充质干细胞（MSC），分析了这种纹理化钛合金表面在成骨分化过程中对细胞黏附的影响。对细胞毒性、细胞活力和分化进行了分析。免疫荧光染色表明，与在扩增培养基中的MSC相比，在成骨诱导培养基中的MSC会产生诸如β3整合素等黏附蛋白，从而以定向方式与钛合金表面生成的微观结构相连。这些结果对于开发具有改善细胞反应的定制钛合金植入物表面具有重要意义。
