Department of Odontostomatological, Orthodontic and Surgical Disciplines, Second University of Naples, Naples, ItalyDepartment of Biochemistry and Biophysics "F. Cedrangolo", Second University of Naples, Naples, ItalyInstitute for Composite and Biomedical Materials, National Research Council (IMCB-CNR), Portici, Italy.
Clin Oral Implants Res. 2012 Jun;23(6):733-740. doi: 10.1111/j.1600-0501.2011.02194.x. Epub 2011 Jun 17.
The aim of this study was to analyse the topographic features of a novel nano-structured oxidized titanium implant surface and to evaluate its effect on the response of human bone marrow mesenchymal stem cells (BM-MSC) compared with a traditional turned surface.
The 10 × 10 × 1 mm turned (control) and oxidized (test) titanium samples (P.H.I. s.r.l.) were examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM) and characterized by height, spatial and hybrid roughness parameters at different dimensional ranges of analysis. Primary cultures of BM-MSC were seeded on titanium samples and cell morphology, adhesion, proliferation and osteogenic differentiation, in terms of alkaline phosphatase activity, osteocalcin synthesis and extracellular matrix mineralization, were evaluated.
At SEM and AFM analyses turned samples were grooved, whereas oxidized surfaces showed a more complex micro- and nano-scaled texture, with higher values of roughness parameters. Cell adhesion and osteogenic parameters were greater on oxidized (P<0.05 at least) vs. turned surfaces, whereas the cell proliferation rate was similar on both samples.
Although both control and test samples were in the range of average roughness proper of smooth surfaces, they exhibited significantly different topographic properties in terms of height, spatial and, mostly, of hybrid parameters. This different micro- and nano-structure resulted in an enhanced adhesion and differentiation of cells plated onto the oxidized surfaces.
本研究旨在分析新型纳米结构化氧化钛种植体表面的地形特征，并评估其对人骨髓间充质干细胞（BM-MSC）反应的影响，与传统的车削表面相比。
采用扫描电子显微镜（SEM）和原子力显微镜（AFM）对 10×10×1mm 车削（对照）和氧化（试验）钛样品（P.H.I. s.r.l.）进行了检查，并在不同的分析维度范围内对其高度、空间和混合粗糙度参数进行了表征。将 BM-MSC 的原代培养物接种在钛样品上，并评估细胞形态、黏附、增殖和成骨分化，以碱性磷酸酶活性、骨钙素合成和细胞外基质矿化来表示。
在 SEM 和 AFM 分析中，车削样品呈凹槽状，而氧化表面显示出更复杂的微纳尺度纹理，具有更高的粗糙度参数值。与车削表面相比，氧化表面的细胞黏附和成骨参数更大（至少 P<0.05），而两种样品的细胞增殖率相似。
尽管对照和试验样品的平均粗糙度均在光滑表面的范围内，但在高度、空间以及主要是混合参数方面，它们表现出明显不同的地形特性。这种不同的微观和纳米结构导致细胞在氧化表面上的黏附和分化增强。
