Dong Ziqing, Peng Zhangsong, Chang Qiang, Zhan Weiqing, Zeng Zhaowei, Zhang Shengchang, Lu Feng
Guangzhou, People's Republic of China From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University.
Plast Reconstr Surg. 2015 Mar;135(3):556e-567e. doi: 10.1097/PRS.0000000000000965.
The major drawback of adipose grafting is its clinical unpredictability, which leads to surgeon and patient dissatisfaction. The mechanisms underlying angiogenesis and regeneration of the graft tissue are still unclear.
Mouse adipose tissue was processed using two different methods (fragmental and integral) and was used to identify the mode of angiogenesis of the graft. Cross-grafting of tissue from normal mice and transgenic mice expressing green fluorescent protein was used to observe the origin of cells during the adipose regeneration.
Almost all the CD31 endothelial cells of the new vessels were derived from the recipient. The new vessels in the graft were mainly formed through recipient vessels growing into the graft rather than the reassembly of donor endothelial cells or the reconnection of recipient and donor vessels. Angiogenesis depends largely on recipient-site environment. The retention of donor-derived tissue dropped to only 10 percent 8 weeks after grafting, and the majority of the key regeneration cells, the CD34 cells, came from the recipient during adipogenesis (p < 0.05). In total, the retention of the recipient-derived tissue was up to 73 percent in the fragmental group and 47.5 percent in the integral group.
The angiogenesis of the graft occurs by the classic "vessel branching" mode, in which the recipient plays a dominant role. The mode of graft tissue retention primarily involves CD34 adipose precursor cells derived from the recipient.
脂肪移植的主要缺点是其临床不可预测性，这导致外科医生和患者都不满意。移植组织血管生成和再生的潜在机制仍不清楚。
采用两种不同方法（碎片法和整体法）处理小鼠脂肪组织，以确定移植的血管生成模式。使用正常小鼠和表达绿色荧光蛋白的转基因小鼠的组织进行交叉移植，以观察脂肪再生过程中细胞的来源。
几乎所有新血管的CD31内皮细胞均来自受体。移植中的新血管主要通过受体血管长入移植组织形成，而非供体内皮细胞重新组装或受体与供体血管重新连接。血管生成在很大程度上取决于受体部位的环境。移植后8周，供体来源组织的留存率降至仅10%，并且在脂肪生成过程中，大多数关键再生细胞即CD34细胞来自受体（p<0.05）。总体而言，碎片组中受体来源组织的留存率高达73%，整体组中为47.5%。
移植的血管生成通过经典的“血管分支”模式发生，其中受体起主导作用。移植组织留存模式主要涉及来自受体的CD34脂肪前体细胞。
