Simanovsky S A, Matveevsky S N, Iordanskaya I V, Spangenberg V E, Kolomiets O L, Bogdanov Yu B
Genetika. 2014 Oct;50(10):1250-3.
After completing their functioning, synaptonemal complexes (SCs) degrade during the diplotene stage. In the pollen mother cells of rye Secale cereal L., this occurs through the formation of gaps in lateral elements of the SCs and the shortening of fragments of SCs until their complete disappearance. However, when contrasting SCs with silver nitrate solution at a pH of 3.5-4.5, these gaps appear to be filled with threads associated with SC lateral elements. As the diplotene stage proceeds and gradual degradation of SC fragments continues, these threads turn into submicroscopic spirals. In this study, we found that the threads and spirals associated with degrading synaptonemal complexes are stained by antibodies to the ASY1 protein ofArabidopsis thaliana lateral elements and thus are degradation products of the lateral elements of SCs.
在完成其功能后，联会复合体（SCs）在双线期阶段降解。在黑麦（Secale cereal L.）的花粉母细胞中，这一过程通过SCs侧元件中形成间隙以及SCs片段缩短直至完全消失来实现。然而，当在pH值为3.5 - 4.5的条件下用硝酸银溶液对比SCs时，这些间隙似乎被与SCs侧元件相关的细丝填满。随着双线期阶段的推进以及SCs片段的逐渐降解持续进行，这些细丝会变成亚显微螺旋结构。在本研究中，我们发现与降解的联会复合体相关的细丝和螺旋结构可被抗拟南芥（Arabidopsis thaliana）侧元件ASY1蛋白的抗体染色，因此它们是SCs侧元件的降解产物。
