Department of Obstetrics and Gynecology University of Michigan, Ann Arbor, Michigan, USA.
Semin Reprod Med. 2012 Jun;30(3):214-21. doi: 10.1055/s-0032-1311523. Epub 2012 May 14.
The growth of mammalian embryos in the laboratory has progressed nicely over the last 60 to 70 years. This has been important for basic studies of preimplantation embryo regulation of growth, development, and differentiation. It has also been critical for the establishment of assisted reproductive technologies in the clinical setting to treat infertility. During the first 40 to 50 years, the primary focus was on defining soluble media components, which fortunately has yielded improved success in the culture of embryos. More recently, attention has shifted to other aspects of culture beyond media composition. In this review we discuss recent advances in preimplantation embryo culture that deal with modifying the insoluble culture environment, producing culture environments that are dynamic and not static, systems that allow more precise gas phase and/or media pH regulation/monitoring, and platforms that integrate with culture systems to allow analysis of embryos and thus assist in selection of the embryos with the greatest implantation potential.
在过去的 60 到 70 年中，哺乳动物胚胎在实验室中的生长取得了很好的进展。这对于研究着床前胚胎生长、发育和分化的调节具有重要意义。它对于建立辅助生殖技术在临床环境中治疗不孕不育症也至关重要。在最初的 40 到 50 年中，主要的重点是确定可溶性培养基成分，这幸运地提高了胚胎培养的成功率。最近，人们的注意力已经转移到了培养基组成之外的培养的其他方面。在这篇综述中，我们讨论了胚胎培养的最新进展，包括修改不溶性培养环境、产生动态而非静态的培养环境、允许更精确地调节/监测气相和/或培养基 pH 值的系统，以及与培养系统集成以允许对胚胎进行分析并从而帮助选择具有最大植入潜力的胚胎的平台。
