Schmidt Deborah, Schuhmacher Frank, Geissner Andreas, Seeberger Peter H, Pfrengle Fabian
Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam (Germany); Freie Universität Berlin, Institute of Chemistry and Biochemistry, Arnimallee 22, 14195 Berlin (Germany).
Chemistry. 2015 Apr 7;21(15):5709-13. doi: 10.1002/chem.201500065. Epub 2015 Feb 26.
Monoclonal antibodies that recognize plant cell wall glycans are used for high-resolution imaging, providing important information about the structure and function of cell wall polysaccharides. To characterize the binding epitopes of these powerful molecular probes a library of eleven plant arabinoxylan oligosaccharides was produced by automated solid-phase synthesis. Modular assembly of oligoarabinoxylans from few building blocks was enabled by adding (2-naphthyl)methyl (Nap) to the toolbox of orthogonal protecting groups for solid-phase synthesis. Conjugation-ready oligosaccharides were obtained and the binding specificities of xylan-directed antibodies were determined on microarrays.
识别植物细胞壁聚糖的单克隆抗体用于高分辨率成像，可提供有关细胞壁多糖结构和功能的重要信息。为了表征这些强大分子探针的结合表位，通过自动化固相合成制备了一个包含11种植物阿拉伯木聚糖寡糖的文库。通过将（2-萘基）甲基（Nap）添加到固相合成的正交保护基团工具箱中，实现了由少量构建模块模块化组装低聚阿拉伯木聚糖。获得了可用于偶联的寡糖，并在微阵列上测定了木聚糖定向抗体的结合特异性。
