Toxicity of chlorophyllin in different wavelengths of visible light against Fasciola gigantica larvae.

Toxicity of chlorophyllin against redia and cercaria larvae of Fasciola gigantica was studied under irradiation of visible light. Highest and lowest toxicity of chlorophyllin against both larvae was noted under red (redia - 8 h LC₅₀ 7.88 mg/10 mL and cercaria - 11.99 mg/10 mL) and green (redia - 8 h LC₅₀ 32.12 mg/10 mL and cercaria - 8 h LC₅₀ 43.80 mg/10 mL) light irradiation respectively. The highest toxicity of chlorophyllin under red light irradiation against redia (8h LC₅₀ 7.88 mg/10 mL)/cercaria (8h LC₅₀ 11.99 mg/10 mL) was followed by white (8 h LC₅₀ redia - 20.48 mg/10 mL, 8 h LC₅₀ cercaria - 18.0 3mg/10 mL), blue (8 h LC₅₀ redia - 33.10 mg/10 mL/ 8 h LC₅₀ cercaria - 19.98 mg/10 mL) and yellow (8 h LC₅₀ redia - 23.87 mg/10 mL/ 8 h LC₅₀ cercaria - 23.48 mg/10 mL). Chlorophyllin treatment in darkness (control I) and without treatment of chlorophyllin, while all other conditions were same as treatment group (control II) caused no mortality of redia/cercaria larva. Chlorophyllin might be a promising new safe strategy to replace synthetic larvicide in fasciolosis control programme.