Sunita Kumari, Kumar Pradeep, Singh Vinay Kumar, Singh Dinesh Kumar
Rev Inst Med Trop Sao Paulo. 2013 Sep-Oct;55(5):303-8. doi: 10.1590/S0036-46652013000500002.
A food-borne trematode infection fascioliasis is one among common public health problems worldwide. It caused a great economic loss for the human race. Control of snail population below a certain threshold level is one of the important methods in the campaign to reduce the incidence of fascioliasis. The life cycle of the parasite can be interrupted by killing the snail or Fasciola larva redia and cercaria inside of the snail Lymnaea acuminata. In vitro toxicity of different binary combinations (1:1 ratio) of plant-derived larvicidal active components such as citral, ferulic acid, umbelliferone, azadirachtin and allicin against Fasciola redia and cercaria were tested. The mortality of larvae was observed at 2h, 4h, 6h and 8h of treatment. In in vitro condition azadirachtin + allicin (1:1 ratio) was highly toxic against redia and cercaria (8h LC50 0.006 and 0.005 mg/L). Toxicity of citral + ferulic acid was lowest against redia and cercaria larvae.
食源性吸虫感染——肝片吸虫病是全球常见的公共卫生问题之一。它给人类造成了巨大的经济损失。将蜗牛数量控制在一定阈值水平以下是降低肝片吸虫病发病率运动中的重要方法之一。寄生虫的生命周期可以通过杀死蜗牛或蜗牛（尖膀胱螺）体内的肝片吸虫幼虫雷蚴和尾蚴来中断。测试了柠檬醛、阿魏酸、伞形酮、印楝素和大蒜素等植物源杀幼虫活性成分不同二元组合（1:1比例）对肝片吸虫雷蚴和尾蚴的体外毒性。在处理2小时、4小时、6小时和8小时时观察幼虫的死亡率。在体外条件下，印楝素+大蒜素（1:1比例）对雷蚴和尾蚴具有高毒性（8小时半数致死浓度分别为0.006和0.005毫克/升）。柠檬醛+阿魏酸对雷蚴和尾蚴幼虫的毒性最低。
