Molinar-Toribio Eunice, Pérez-Jiménez Jara, Ramos-Romero Sara, Romeu Marta, Giralt Montserrat, Taltavull Núria, Muñoz-Cortes Mònica, Jáuregui Olga, Méndez Lucía, Medina Isabel, Torres Josep Lluís
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC),Barcelona,Spain.
Unidad de Farmacología, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili,Reus,Spain.
Br J Nutr. 2015 Mar 28;113(6):878-87. doi: 10.1017/S0007114514004437. Epub 2015 Feb 27.
The increasing incidence of the metabolic syndrome (MetS), a combination of risk factors before the onset of CVD and type 2 diabetes, encourages studies on the role of functional food components such as long-chain n-3 PUFA as preventive agents. In the present study, we explore the effect of EPA and DHA supplementation in different proportions on spontaneously hypertensive obese (SHROB) rats, a model for the MetS in a prediabetic state with mild oxidative stress. SHROB rats were randomised into four groups (n 7), each supplemented with EPA/DHA at ratios of 1:1, 2:1 and 1:2, or soyabean oil as the control for 13 weeks. The results showed that in all the proportions tested, EPA/DHA supplementation significantly lowered total and LDL-cholesterol concentrations, compared with those of the control group. EPA/DHA supplementation at the ratios of 1:1 and 2:1 significantly decreased inflammation (C-reactive protein levels) and lowered oxidative stress (decreased excretion of urinary isoprostanes), mainly at the ratio of 1:2. The activity of antioxidant enzymes increased in erythrocytes, abdominal fat and kidneys, with magnitudes depending on the EPA:DHA ratio. PUFA mixtures from fish affected different MetS markers of CVD risk factors in SHROB rats, depending on the ratios of EPA/DHA supplementation. The activation of endogenous defence systems may be related to the reduction of inflammation and oxidative stress.
代谢综合征（MetS）是心血管疾病（CVD）和2型糖尿病发病前多种风险因素的组合，其发病率不断上升，这促使人们对长链n-3多不饱和脂肪酸（PUFA）等功能性食品成分作为预防剂的作用进行研究。在本研究中，我们探讨了不同比例补充二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）对自发性高血压肥胖（SHROB）大鼠的影响，SHROB大鼠是处于轻度氧化应激的糖尿病前期状态下代谢综合征的模型。将SHROB大鼠随机分为四组（每组n = 7），分别以1:1、2:1和1:2的比例补充EPA/DHA，或以大豆油作为对照，持续13周。结果表明，在所有测试比例下，与对照组相比，补充EPA/DHA均显著降低了总胆固醇和低密度脂蛋白胆固醇浓度。以1:1和2:1的比例补充EPA/DHA可显著减轻炎症（C反应蛋白水平）并降低氧化应激（尿异前列腺素排泄减少），主要是在1:2的比例下。红细胞、腹部脂肪和肾脏中抗氧化酶的活性增加，其幅度取决于EPA:DHA的比例。根据补充EPA/DHA的比例，来自鱼类的多不饱和脂肪酸混合物会影响SHROB大鼠中不同的心血管疾病风险因素的代谢综合征标志物。内源性防御系统的激活可能与炎症和氧化应激的减轻有关。
