Thorns C J, Wells G A, Morris J A, Bridges A, Higgins R
Central Veterinary Laboratory New Haw, Weybridge, Surrey, Gt. Britain.
Vet Microbiol. 1989 Aug;20(4):377-81. doi: 10.1016/0378-1135(89)90062-x.
A panel of monoclonal antibodies against fimbrial adhesins of porcine enterotoxigenic Escherichia coli were evaluated for the detection of enteric colibacillosis in paraffin-wax embedded sections of piglet small intestine. Using the immunoperoxidase technique, monoclonal antibodies were used to detect epitopes on the K99 adhesin and on the a and c regions of the K88 adhesin. However, monoclonal antibodies to the F41 and 987P adhesins failed to react in sections with organisms colonising the intestine of gnotobiotic piglets monoinfected with strains bearing those adhesins, whereas corresponding polyclonal antisera gave positive results. In contrast to apparent expression of all K99 organisms, only a proportion of organisms were identified by monoclonal or polyclonal antibodies as expressing K88. In some instances, failure of immunostaining was attributed to prolonged storage of tissue in formalin.
对一组针对猪产肠毒素大肠杆菌菌毛粘附素的单克隆抗体进行了评估，以检测仔猪小肠石蜡包埋切片中的肠型大肠杆菌病。采用免疫过氧化物酶技术，使用单克隆抗体检测K99粘附素以及K88粘附素的a和c区域上的表位。然而，针对F41和987P粘附素的单克隆抗体未能在切片中与定殖于无菌仔猪肠道的、携带这些粘附素的菌株发生反应，而相应的多克隆抗血清给出了阳性结果。与所有K99菌的明显表达情况相反，只有一部分菌被单克隆或多克隆抗体鉴定为表达K88。在某些情况下，免疫染色失败归因于组织在福尔马林中的长期保存。
