多发性硬化症患者的疲劳：它与促炎和抗炎细胞因子有关吗？
Fatigue in patients with multiple sclerosis: is it related to pro- and anti-inflammatory cytokines?
作者信息
Malekzadeh Arjan, Van de Geer-Peeters Wietske, De Groot Vincent, Teunissen Charlotte Elisabeth, Beckerman Heleen
机构信息
Neurochemistry Laboratory, Department of Clinical Chemistry, VU University Medical Center, Neuroscience Campus Amsterdam, P.O. Box 7057, 1007 MB Amsterdam, Netherlands ; MS Center Amsterdam, P.O. Box 7057, 1007 MB Amsterdam, Netherlands.
Department of Rehabilitation Medicine, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, Netherlands.
出版信息
Dis Markers. 2015;2015:758314. doi: 10.1155/2015/758314. Epub 2015 Jan 19.
OBJECTIVE
To investigate the pathophysiological role of pro- and anti-inflammatory cytokines in primary multiple sclerosis-related fatigue.
METHODS
Fatigued and non-fatigued patients with multiple sclerosis (MS) were recruited and their cytokine profiles compared. Patients with secondary fatigue were excluded. Fatigue was assessed with the self-reported Checklist Individual Strength (CIS20r), subscale fatigue. A CIS20r fatigue cut-off score of 35 was applied to differentiate between non-fatigued (CIS20r fatigue ≤34) and fatigued (CIS20r fatigue ≥35) patients with MS. Blood was collected to determine the serum concentrations of pro-inflammatory cytokines (IL-1β, IL-2, IL-6, IL-8, IL-12p70, IL-17, TNFα, and IFN-γ) and anti-inflammatory cytokines (IL-4, IL-5, IL-10, and IL-13). We controlled for the confounding effect of age, gender, duration of MS, disease severity, type of MS, and use of immunomodulatory drugs.
RESULTS
Similar cytokine levels were observed between MS patients with (n = 21) and without fatigue (n = 14). Adjusted multiple regression analyses showed a single significant positive relationship, that of IL-6 with CIS20r fatigue score. The explained variance of the IL-6 model was 21.1%, once adjusted for the confounding effect of age.
CONCLUSION
The pro-inflammatory cytokine interleukin-6 (IL-6) may play a role in the pathophysiology of primary fatigue in patients with MS.
TRIAL REGISTRATIONS
ISRCTN69520623, ISRCTN58583714, and ISRCTN82353628.
目的
研究促炎细胞因子和抗炎细胞因子在原发性多发性硬化相关疲劳中的病理生理作用。
方法
招募患有疲劳和无疲劳的多发性硬化症（MS）患者，并比较他们的细胞因子谱。排除继发性疲劳患者。使用自我报告的个人力量检查表（CIS20r）疲劳分量表评估疲劳。应用CIS20r疲劳临界值35分来区分无疲劳（CIS20r疲劳≤34）和有疲劳（CIS20r疲劳≥35）的MS患者。采集血液以测定促炎细胞因子（IL-1β、IL-2、IL-6、IL-8、IL-12p70、IL-17、TNFα和IFN-γ）和抗炎细胞因子（IL-4、IL-5、IL-10和IL-13）的血清浓度。我们控制了年龄、性别、MS病程、疾病严重程度、MS类型和免疫调节药物使用的混杂效应。
结果
在有疲劳（n = 21）和无疲劳（n = 14）的MS患者之间观察到相似的细胞因子水平。调整后的多元回归分析显示了一种单一的显著正相关关系，即IL-6与CIS20r疲劳评分的关系。一旦对年龄的混杂效应进行调整，IL-6模型的解释方差为21.1%。
结论
促炎细胞因子白细胞介素-6（IL-6）可能在MS患者原发性疲劳的病理生理过程中起作用。
试验注册号
ISRCTN69520623、ISRCTN58583714和ISRCTN82353628。
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