Dogra Mitu, Sidhu Meena, Vasudev Rahul, Dogra Ashu
Department of Immunohematology and Transfusion Medicine, GMC, Jammu, Jammu and Kashmir, India.
Asian J Transfus Sci. 2015 Jan-Jun;9(1):6-8. doi: 10.4103/0973-6247.150936.
The aim of this study was to analyse and compare the activity of factor V, VIII and fibrinogen level in fresh frozen plasma and frozen plasma frozen after 8 hrs but within 24 hours after phlebotomy.
Fresh frozen plasma separated from whole blood within 8 hours was compared with plasma separated within 24 hours after phlebotomy in terms of coagulation factors V and VIII and level of fibrinogen by standard methods using semi automated coagulometer sysmex CA50.
Longer storage of whole blood before processing resulted in significant decrease (18.4%) in activity of factor VIII but the fall in activity of factor V (6.52%) or level of fibrinogen (1.81%) was not significant.
These data suggest that there is good retention of coagulation factors in both types of plasma. Although there is significant fall in activity of factor VIII, but it is an acute phase reactant and raised in most of the diseases so it is suggested that frozen plasma would be an acceptable product for most patients requiring fresh frozen plasma.
本研究的目的是分析和比较新鲜冰冻血浆以及采血后8小时但在24小时内冷冻的冰冻血浆中因子V、VIII的活性和纤维蛋白原水平。
采用半自动凝血分析仪sysmex CA50，通过标准方法，比较了8小时内从全血中分离出的新鲜冰冻血浆与采血后24小时内分离出的血浆中凝血因子V和VIII以及纤维蛋白原水平。
处理前全血储存时间延长导致因子VIII活性显著下降（18.4%），但因子V活性下降（6.52%）或纤维蛋白原水平下降（1.81%）不显著。
这些数据表明两种类型的血浆中凝血因子保留良好。虽然因子VIII活性显著下降，但它是一种急性期反应物，在大多数疾病中会升高，因此建议对于大多数需要新鲜冰冻血浆的患者，冰冻血浆是一种可接受的产品。
Zotero 插件