Department of Blood Bank, Tepecik Educational and Research Hospital, Izmir, Turkey.
Blood Transfus. 2012 Apr;10(2):191-3. doi: 10.2450/2011.0050-11. Epub 2011 Nov 16.
The aim of this study was to assess whether the quantities of factor VIII and factor IX in fresh-frozen plasma produced from whole blood stored at 4 °C for 24 hours are adequate for their intended purpose.
Fresh-frozen plasma separated from whole blood after storage at 4 °C overnight (24 hours from donation) was compared with plasma prepared 8 hours after donation using a standard method. The amounts of factor VIII and factor IX obtained with the two methods were compared.
Compared to the levels of factor VIII and factor IX in plasma prepared within 8 hours of blood collection, the levels in plasma prepared after 24 hours of storage at 4 °C were 25% and 9% lower, respectively. Ninety percent of the factor VIII and 100% of the factor IX levels were above 0.5 IU/mL (standard haematology reference range) after 24 hours of storage.
These data suggest that there is good retention of coagulation factor activity in plasma produced from whole blood stored at 4 ºC for 24 hours and that such plasma would be an acceptable product for most patients requiring fresh-frozen plasma.
本研究旨在评估在 4°C 储存 24 小时的全血中制备的新鲜冷冻血浆中的因子 VIII 和因子 IX 的量是否足以达到预期目的。
将在 4°C 过夜（从捐献开始 24 小时）储存后的全血分离出的新鲜冷冻血浆与使用标准方法在捐献后 8 小时制备的血浆进行比较。比较两种方法获得的因子 VIII 和因子 IX 的量。
与在采集血液后 8 小时内制备的血浆中的因子 VIII 和因子 IX 水平相比，在 4°C 储存 24 小时后的血浆中，因子 VIII 和因子 IX 的水平分别降低了 25%和 9%。在储存 24 小时后，90%的因子 VIII 和 100%的因子 IX 水平高于 0.5IU/mL（标准血液学参考范围）。
这些数据表明，在 4°C 储存 24 小时的全血中制备的新鲜冷冻血浆中凝血因子活性保留良好，对于大多数需要新鲜冷冻血浆的患者来说，这种血浆是一种可接受的产品。
