Seroogy K, Hökfelt T, Buchan A, Brown J C, Terenius L, Norman A W, Goldstein M
Department of Physiology, University of North Carolina, Chapel Hill 27599.
Brain Res. 1989 Sep 4;496(1-2):389-96. doi: 10.1016/0006-8993(89)91095-0.
A double-labeling immunofluorescence colocalization technique was used to examine the extent of coexistence of somatostatin (SOM)-like immunoreactivity with neuropeptide Y (NPY)-, tyrosine hydroxylase (TH)- and vitamin D-dependent calcium binding protein (D-CaBP)-like immunoreactivities in neurons of the rat main olfactory bulb. SOM-like immunoreactivity (SOM-I) was distributed within restricted populations of periglomerular neurons and deep short-axon cells, and rarely within superficial short-axon cells at the glomerular layer/external plexiform layer (GL/EPL) border region. Double-labeling analysis revealed that all of the SOM-I deep and superficial short-axon cells also contained NPY-I. Colocalization of SOM-I and TH-I or of SOM-I and D-CaBP-I was infrequently observed within periglomerular neurons. The rare SOM-I short-axon cells at the GL/EPL border always exhibited D-CaBP-I. These results demonstrate virtual complete coexistence of SOM and NPY in short-axon neurons of the main olfactory bulb. With a few exceptions, however, bulbar SOM neurons appear to constitute subclasses of periglomerular cells immunohistochemically distinct from those containing TH or D-CaBP.
采用双标免疫荧光共定位技术，检测大鼠主嗅球神经元中生长抑素（SOM）样免疫反应性与神经肽Y（NPY）、酪氨酸羟化酶（TH）和维生素D依赖性钙结合蛋白（D-CaBP）样免疫反应性共存的程度。SOM样免疫反应性（SOM-I）分布于肾小球周围神经元和深层短轴突细胞的特定群体中，在肾小球层/外丛状层（GL/EPL）边界区域的浅层短轴突细胞中很少见。双标分析显示，所有SOM-I深层和浅层短轴突细胞也含有NPY-I。在肾小球周围神经元中很少观察到SOM-I与TH-I或SOM-I与D-CaBP-I的共定位。GL/EPL边界处罕见的SOM-I短轴突细胞总是表现出D-CaBP-I。这些结果表明，SOM和NPY在主嗅球的短轴突神经元中几乎完全共存。然而，除了少数例外，嗅球SOM神经元似乎构成了肾小球周围细胞的亚类，在免疫组织化学上与含有TH或D-CaBP的细胞不同。
