Gall C, Seroogy K B, Brecha N
Brain Res. 1986 May 28;374(2):389-94. doi: 10.1016/0006-8993(86)90436-1.
The distribution of vasoactive intestinal peptide (VIP)- and neuropeptide Y (NPY)-like immunoreactivities in the Sprague-Dawley rat main olfactory bulb was analyzed using the peroxidase-antiperoxidase light microscopic immunocytochemical technique. VIP-like immunoreactivity was most prominently localized within a large number of intermediate-sized neurons whose perikarya and extensively branched varicose processes remained confined to the external plexiform layer (EPL). A few small short-axon type neurons in the mitral cell layer and granule cell layer (GRL) and even fewer large neurons in the glomerular layer (GL)/EPL border region contained immunoreactivity for VIP as well. Neuropeptide Y-like immunoreactivity (NPY-I) was principally localized within sparsely distributed large multipolar neurons of the deep GRL and within axons distributed with diminishing density from deep to superficial GRL. In addition, dense NPY-I was localized within very few large superficial short-axon type neurons of the GL/EPL border region. The restricted laminar and cellular distribution of NPY-I and VIP-I suggests that both peptides may act to modulate granule cell activity, and therefore, indirectly, olfactory bulb output.
采用过氧化物酶-抗过氧化物酶光镜免疫细胞化学技术，分析了血管活性肠肽（VIP）和神经肽Y（NPY）样免疫反应性在Sprague-Dawley大鼠主嗅球中的分布情况。VIP样免疫反应性最显著地定位于大量中等大小的神经元内，其胞体和广泛分支的曲张状突起局限于外丛状层（EPL）。在僧帽细胞层和颗粒细胞层（GRL）中有一些小的短轴突型神经元，在肾小球层（GL）/EPL边界区域有更少的大神经元也含有VIP免疫反应性。神经肽Y样免疫反应性（NPY-I）主要定位于GRL深部稀疏分布的大多极神经元内，以及从GRL深部到浅部分布密度逐渐降低的轴突内。此外，密集的NPY-I定位于GL/EPL边界区域极少数大的浅部短轴突型神经元内。NPY-I和VIP-I在层和细胞分布上的局限性表明，这两种肽可能都起到调节颗粒细胞活性的作用，因此间接地调节嗅球输出。
