Zhang Ti, Cui Huizhong, Fang Chia-Yi, Cheng Kun, Yang Xinmai, Chang Huan-Cheng, Forrest M Laird
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66047, USA.
Nanomedicine (Lond). 2015 Mar;10(4):573-87. doi: 10.2217/nnm.14.141.
The aim is to develop irradiated nanodiamonds (INDs) as a molecularly targeted contrast agent for high-resolution and phenotype-specific detection of breast cancer with photoacoustic (PA) imaging. The surface of acid treated radiation-damaged nanodiamonds was grafted with PEG to improve its stability and circulation time in blood, followed by conjugation to an anti-HER2 peptide with a final nanoparticle size of approximately 92 nm. Immunocompetent mice bearing orthotopic HER2-positive or negative tumors were administered INDs and PA imaged using an 820-nm near-infrared laser. PA images demonstrated that INDs accumulate in tumors and completely delineated the entire tumor within 10 h. HER2 targeting significantly enhanced imaging of HER2-positive tumors. Pathological examination demonstrated INDs are nontoxic. PA technology is adaptable to low-cost bedside medicine, and with new contrast agents described herein, PA can achieve high-resolution (sub-mm) and phenotype-specific monitoring of cancer growth.
目的是开发辐照纳米金刚石（INDs）作为一种分子靶向造影剂，用于通过光声（PA）成像对乳腺癌进行高分辨率和表型特异性检测。对经酸处理的辐射损伤纳米金刚石表面进行聚乙二醇（PEG）接枝，以提高其在血液中的稳定性和循环时间，随后与抗HER2肽偶联，最终纳米颗粒尺寸约为92nm。对携带原位HER2阳性或阴性肿瘤的免疫活性小鼠给予INDs，并使用820nm近红外激光进行PA成像。PA图像显示INDs在肿瘤中积聚，并在10小时内完全勾勒出整个肿瘤。HER2靶向显著增强了HER2阳性肿瘤的成像。病理检查表明INDs无毒。PA技术适用于低成本的床边医学，并且使用本文所述的新型造影剂，PA可以实现对癌症生长的高分辨率（亚毫米）和表型特异性监测。
