Fulawka Lukasz, Donizy Piotr, Halon Agnieszka
Department of Pathomorphology and Oncological Cytology, Wroclaw Medical University, ul. Borowska 213, Wroclaw, 50-556, Poland.
Department of Pathomorphology, Lower Silesian Oncology Centre, pl. Hirszfelda 12, Wroclaw, 53-413, Poland.
Biol Res. 2014 Dec 10;47(1):66. doi: 10.1186/0717-6287-47-66.
As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew and produce a heterogeneous tumour cell population are cancer stem cells. CSCs probably derive from normal stem cells, although progenitor cells may be taken into consideration as the source of cancer stem cells. CSCs reside in the niche defined as the microenvironment formed by stromal cells, vasculature and extracellular matrix. The CSC assays include FACS sorting, xenotransplantation to immunodeficient mice (SCID), incubation with Hoechst 33342 dye, cell culture in non-adherent conditions, cell culture with bromodeoxyuridine. CSCs have certain properties that make them resistant to anticancer therapy, which suggests they may be the target for potential therapeutic strategies.
就其形态学和生物学而言，肿瘤由异质性细胞群体组成。癌症干细胞（CSC）假说认为，肿瘤是分层组织的，并非所有细胞都同样有能力产生后代，这与正常组织类似。唯一能够自我更新并产生异质性肿瘤细胞群体的细胞是癌症干细胞。癌症干细胞可能源自正常干细胞，尽管祖细胞也可能被视为癌症干细胞的来源。癌症干细胞存在于被定义为由基质细胞、脉管系统和细胞外基质形成的微环境的生态位中。癌症干细胞检测方法包括荧光激活细胞分选（FACS）、移植到免疫缺陷小鼠（SCID）体内、用Hoechst 33342染料孵育、在非贴壁条件下进行细胞培养、用溴脱氧尿苷进行细胞培养。癌症干细胞具有某些使其对抗癌治疗产生抗性的特性，这表明它们可能是潜在治疗策略的靶点。
