School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
Henan Key Laboratory of Bioactive Macromolecules, Zhengzhou 450001, China.
Int J Mol Sci. 2021 Oct 28;22(21):11707. doi: 10.3390/ijms222111707.
SUMOylation and ubiquitylation are homologous processes catalyzed by homologous enzymes, and they are involved in nearly all aspects of eukaryotic biology. Planarians, which have the remarkable ability to regenerate their central nervous system (CNS), provide an excellent opportunity to investigate the molecular processes of CNS regeneration in vivo. In this study, we analyzed gene expression profiles during head regeneration with an RNA-seq-based screening approach and found that and were required for head regeneration in planarians. RNA interference targeting of caused the phospho-H3 mitotic cells to decrease in quantity, or even become absent as a part of the RNAi phenotype, which also showed the collapse of the stem cell lineage along with the reduced expression of epidermal differentiation markers. Furthermore, we found that RNAi induced increased cell division and promoted the premature differentiation during regeneration. Taken together, our findings show that and are required for stem cell maintenance in the planarian , which helps to elucidate the role of SUMOylation and ubiquitylation in regulating the regeneration process.
SUMOylation 和泛素化是由同源酶催化的同源过程，它们几乎涉及真核生物生物学的各个方面。水螅，具有显著的再生中枢神经系统（CNS）的能力，为在体内研究 CNS 再生的分子过程提供了极好的机会。在这项研究中，我们通过基于 RNA-seq 的筛选方法分析了头部再生过程中的基因表达谱，发现 和 在水螅头部再生中是必需的。针对 的 RNA 干扰靶向导致磷酸化-H3 有丝分裂细胞数量减少，甚至作为 RNAi 表型的一部分消失，这也显示出随着表皮分化标记物表达的降低，干细胞谱系的崩溃。此外，我们发现 RNAi 诱导了细胞分裂的增加，并在再生过程中促进了过早分化。总之，我们的研究结果表明， 和 在水螅 中维持干细胞所必需的，这有助于阐明 SUMOylation 和泛素化在调节再生过程中的作用。
