Ventolini G, Mitchell E, Salazar M
Texas Tech University Health Sciences Center at the Permian Basin, 800 W. 4th Street, Odessa, TX 79763, United States.
Texas Tech University Health Sciences Center at the Permian Basin, 800 W. 4th Street, Odessa, TX 79763, United States.
Med Hypotheses. 2015 May;84(5):417-20. doi: 10.1016/j.mehy.2014.12.020. Epub 2015 Jan 9.
Biofilm formation by nonpathogenic bacteria is responsible for their stable maintenance in vivo ecosystems as it promotes long-term permanence on the host's vaginal mucosa. Biofilm formation by Lactobacilli has been reported in vitro but not in vivo. We hypothesize the presence of biofilm formation in vivo could be also documented by microscope photographs (MP) of wet mounts obtained from uninfected vaginal samples satisfying rigorous scientific identification criteria. We analyzed 400 MP from our database, and we were able to determine that 12 MP from 6 different patients contained clues of the formation of biofilm by Lactobacilli. The most probable lactobacillus involved is presumed to be Lactobacillus jensenii. The documentation of biofilm formation by vaginal Lactobacilli at fresh wet mount preparation is significant and has several important clinical preventive and therapeutic implications.
非致病性细菌形成生物膜有助于它们在体内生态系统中稳定存在，因为这能促进其在宿主阴道黏膜上长期留存。已有体外报道乳杆菌可形成生物膜，但体内情况尚无报道。我们推测，通过满足严格科学鉴定标准的未感染阴道样本湿片显微镜照片（MP），也能够证明体内存在生物膜形成现象。我们分析了数据库中的400张MP，确定来自6名不同患者的12张MP包含乳杆菌形成生物膜的线索。最可能涉及的乳杆菌被推测为詹氏乳杆菌。在新鲜湿片制备时记录阴道乳杆菌形成生物膜具有重要意义，并且具有若干重要的临床预防和治疗意义。
