Singh Ram P, Shafeeque Chathathayil M, Sharma Sanjeev K, Pandey Nitin K, Singh Renu, Mohan Jag, Kolluri Gautham, Saxena Meeta, Sharma Bhaskar, Sastry Kochiganti V H, Kataria Jag M, Azeez Parappurath A
Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Coimbatore, India.
Smithsonian Conservation Biology Institute, Front Royal, Virginia, USA.
Environ Toxicol Chem. 2015 Jul;34(7):1617-22. doi: 10.1002/etc.2957. Epub 2015 Apr 30.
Bisphenol A (BPA) acts as an endocrine disruptor, affects animal reproductive success in vivo and affects sperm functions in vitro at environmentally relevant concentrations, leading to reduction in sperm motility and fertilizing ability in fish. The effect of in vitro BPA on avian sperm functions has not been explored. The present study examined the effect of environmentally relevant concentrations of BPA (0 mM, 0.18 mM, 0.37 mM, and 0.74 mM) on sperm functions in chicken in vitro. Sperm were exposed to concentrations of BPA for 30 min and analyzed for motility, fertilizing ability, live sperm percentage, and mitochondrial membrane potential (Δψm). Results showed that BPA at a concentration of 0.74 mM significantly decreased motility, fertilizing ability, live sperm count percentage, and sperm Δψm. Sperm motility was positively correlated with fertility (r = 0.73, p ≤ 0.01), live sperm percentage (r = 0.64, p ≤ 0.01), and high Δψm (r = 0.44, p ≤ 0.01). A dose-dependent and time-dependent effect of BPA was observed on sperm motility at all BPA concentrations. However, sperm's fertilizing ability was unaffected in low BPA concentration (0.18 mM and 0.37 mM). A significantly higher percentage of moribund sperm was observed at 0.37 mM and 0.74 mM BPA compared with at 0.18 mM BPA, in the negative control, and in the vehicle control. The present study confirms that environmentally relevant concentrations of BPA are capable of compromising sperm functions, leading to reduction in fertilizing ability of chicken sperm.
双酚A（BPA）作为一种内分泌干扰物，在环境相关浓度下会影响动物体内的生殖成功率，并在体外影响精子功能，导致鱼类精子活力和受精能力下降。体外BPA对禽类精子功能的影响尚未得到研究。本研究检测了环境相关浓度的BPA（0 mM、0.18 mM、0.37 mM和0.74 mM）对鸡精子体外功能的影响。将精子暴露于不同浓度的BPA中30分钟，然后分析其活力、受精能力、活精子百分比和线粒体膜电位（Δψm）。结果显示，浓度为0.74 mM的BPA显著降低了精子活力、受精能力、活精子计数百分比和精子Δψm。精子活力与受精率呈正相关（r = 0.73，p≤0.01）、与活精子百分比呈正相关（r = 0.64，p≤0.01）、与高Δψm呈正相关（r = 0.44，p≤0.01）。在所有BPA浓度下均观察到BPA对精子活力有剂量依赖性和时间依赖性影响。然而，低BPA浓度（0.18 mM和0.37 mM）下精子的受精能力未受影响。与0.18 mM BPA、阴性对照和溶剂对照相比，在0.37 mM和0.74 mM BPA浓度下观察到濒死精子的比例显著更高。本研究证实，环境相关浓度的BPA能够损害精子功能，导致鸡精子受精能力下降。
