Wan Xiaofeng, Ru Yanfei, Chu Chen, Ni Zimei, Zhou Yuchuan, Wang Shoulin, Zhou Zuomin, Zhang Yonglian
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China University of Chinese Academy of Sciences, Beijing 100864, China.
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Acta Biochim Biophys Sin (Shanghai). 2016 Jun;48(6):573-80. doi: 10.1093/abbs/gmw039. Epub 2016 May 12.
Bisphenol A (BPA) is a synthetic estrogen-mimic chemical. It has been shown to affect many reproductive endpoints. However, the effect of BPA on the mature sperm and the mechanism of its action are not clear yet. Here, our in vitro studies indicated that BPA could accelerate sperm capacitation-associated protein tyrosine phosphorylation in time- and dose-dependent manners. In vivo, the adult male rats exposed to a high dose of BPA could result in a significant increase in sperm activity. Further investigation demonstrated that BPA could accelerate capacitation-associated protein tyrosine phosphorylation even if sperm were incubated in medium devoid of BSA, HCO3 (-), and Ca(2+) However, this action of BPA stimulation could be blocked by H89, a highly selective blocker of protein kinase A (PKA), but not by KH7, a specific inhibitor of adenylyl cyclase. These data suggest that BPA may activate PKA to affect sperm functions and male fertility.
双酚A（BPA）是一种合成的雌激素模拟化学物质。已表明它会影响许多生殖终点。然而，BPA对成熟精子的影响及其作用机制尚不清楚。在此，我们的体外研究表明，BPA可呈时间和剂量依赖性地加速精子获能相关的蛋白质酪氨酸磷酸化。在体内，暴露于高剂量BPA的成年雄性大鼠可导致精子活力显著增加。进一步研究表明，即使精子在不含牛血清白蛋白（BSA）、碳酸氢根离子（HCO3 (-)）和钙离子（Ca(2+)）的培养基中孵育，BPA仍可加速获能相关的蛋白质酪氨酸磷酸化。然而，BPA的这种刺激作用可被蛋白激酶A（PKA）的高度选择性阻滞剂H89阻断，但不能被腺苷酸环化酶的特异性抑制剂KH7阻断。这些数据表明，BPA可能通过激活PKA来影响精子功能和雄性生育能力。
