Soleimani Maryam Zohour, Khorsandi Layasadat, Asadi-Fard Yousef, Rezaei-Tazangi Fatemeh, Ashtari Atefeh
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
JBRA Assist Reprod. 2025 Mar 12;29(1):53-60. doi: 10.5935/1518-0557.20240089.
This study aimed to explore the potential protective effects of adipose-derived mesenchymal stem cell secretome (ASE) on oxidative stress triggered by Bisphenol-A (BisA) exposure in testicular mitochondria and sperm quality of rats.
Testicular tissue mitochondria and sperms were exposed to BisA (8 μM) and ASE (50 or 100 μg). ∆Ψm (mitochondrial membrane potential), reactive oxygen species (ROS) levels, antioxidant biomarkers, and sperm parameters were measured.
BisA elevated biomarkers of oxidative stress in mitochondria, while the levels of antioxidant activity and ∆Ψm decreased significantly. BisA harmed the morphology, survival rate, and mobility of the spermatozoids. ASE lowered malondialdehyde contents and ROS generation in the mitochondria, increased ∆Ψm, and reversed sperm quality.
These data indicated that ASE effectively reduced BisA-induced damage to mitochondria and enhanced sperm quality by averting oxidative stress.
本研究旨在探讨脂肪间充质干细胞分泌组（ASE）对双酚A（BisA）暴露引发的大鼠睾丸线粒体氧化应激及精子质量的潜在保护作用。
将睾丸组织线粒体和精子暴露于BisA（8 μM）和ASE（50或100 μg）中。测量线粒体膜电位（∆Ψm）、活性氧（ROS）水平、抗氧化生物标志物和精子参数。
BisA升高了线粒体氧化应激的生物标志物，而抗氧化活性水平和∆Ψm显著降低。BisA损害了精子的形态、存活率和运动能力。ASE降低了线粒体中丙二醛含量和ROS生成，增加了∆Ψm，并改善了精子质量。
这些数据表明，ASE通过避免氧化应激有效减少了BisA诱导的线粒体损伤并提高了精子质量。
