Koväcs P, Csaba G, Nagao S, Nozawa Y
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Microbios. 1989;59(239):123-8.
The primary interaction with insulin accounted for considerable increases in both the calmodulin content and guanylate cyclase activity of Tetrahymena. Both activities were still elevated after 24 h (6-8 generations), but while the calmodulin level showed a decrease, guanylate cyclase activity showed a further significant increase relative to the immediate response. A second treatment with insulin decreased rather than increased both activities, but to dissimilar degrees, in that the calmodulin content returned to the control level, whereas guanylate cyclase activity still increased over the level measured after the first treatment. It appears that insulin imprinting altered the calmodulin-dependent guanylate cyclase regulation in Tetrahymena, and caused a switch-over to an 'energy-saving' system through decelerating the breakdown of cGMP by phosphodiesterase.
胰岛素的初次作用使四膜虫的钙调蛋白含量和鸟苷酸环化酶活性显著增加。24小时（6 - 8代）后，这两种活性仍然升高，但钙调蛋白水平下降，而鸟苷酸环化酶活性相对于即时反应进一步显著增加。第二次用胰岛素处理使这两种活性都降低而非增加，但程度不同，钙调蛋白含量恢复到对照水平，而鸟苷酸环化酶活性仍高于首次处理后测得的水平。似乎胰岛素印记改变了四膜虫中钙调蛋白依赖性鸟苷酸环化酶的调节，并通过减缓磷酸二酯酶对环磷酸鸟苷的分解导致向“节能”系统的转换。
