Kovács P, Csaba G, Ito Y, Nozawa Y
Department of Biology, Semmelwels University of Medicine, Budapest, Hungary.
Biochem Biophys Res Commun. 1996 May 15;222(2):359-61. doi: 10.1006/bbrc.1996.0748.
Insulin treatment of the unicellular Tetrahymena enormously increases the activity of phospholipase D (PLD) within 30 min. Insulin pretreatment (hormonal imprinting) does not influence basal PLD activity after 24 h. The second insulin treatment failed to stimulate PLD activity in insulin imprinted Tetrahymena. The results suggest the presence of the functional PLD activity in Tetrahymena and that hormonal imprinting modifies signal transduction to PLD, as previously reported in calmodulin activation and phophatidylinositol 4,5-bis-phosphate (PIP2) synthesis.
对单细胞四膜虫进行胰岛素处理可在30分钟内极大地提高磷脂酶D（PLD）的活性。胰岛素预处理（激素印记）在24小时后不影响基础PLD活性。第二次胰岛素处理未能刺激经胰岛素印记的四膜虫中的PLD活性。结果表明四膜虫中存在功能性PLD活性，并且激素印记会改变向PLD的信号转导，正如先前在钙调蛋白激活和磷脂酰肌醇4,5-二磷酸（PIP2）合成中所报道的那样。
