从云纹石斑鱼（Sebastiscus marmoratus）中分离新的多态性微卫星标记。

Isolation of new polymorphic microsatellite markers from the marbled rockfish Sebastiscus marmoratus.

作者信息

Deng H W, Li Z B, Dai G, Yuan Y, Ning Y F, Shangguan J B, Huang Y S

机构信息

Fisheries College, Jimei University, Xiamen, China.

Fisheries College, Jimei University, Xiamen, China

出版信息

Genet Mol Res. 2015 Jan 30;14(1):758-62. doi: 10.4238/2015.January.30.19.

DOI:10.4238/2015.January.30.19

PMID:25730013

Abstract

The marbled rockfish, Sebastiscus marmoratus, is an important commercially near-shore fish that inhabits the beach rocky bottom from Japan to the South China Sea. Eleven polymorphic microsatellite loci were developed from S. marmoratus and were used to identify polymorphisms in 30 samples from a wild population. The allele locus number ranged from 2 to 7. Polymorphism data content ranged from 0.032 to 0.751. The observed and expected heterozygosity levels were 0.0333-0.9667 and 0.0328-0.7675, respectively. Two loci, Smd1-112 and Smd2-80, deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers will facilitate further studies of genetic diversity and genetic differentiation of S. marmoratus.

摘要

云纹平鲉（Sebastiscus marmoratus）是一种重要的近岸商业鱼类，栖息于从日本到中国南海的海滩岩礁底部。从云纹平鲉中开发出了11个多态性微卫星位点，并用于鉴定来自野生种群的30个样本中的多态性。等位基因位点数范围为2至7。多态性数据含量范围为0.032至0.751。观察到的和预期的杂合度水平分别为0.0333 - 0.9667和0.0328 - 0.7675。两个位点Smd1 - 112和Smd2 - 80偏离了哈迪-温伯格平衡。这些多态性微卫星标记将有助于进一步研究云纹平鲉的遗传多样性和遗传分化。