Liu H B, Liu S F, Ye J B, Yuan Y J, Ding S X, Zhuang Z M
Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Ocean University, Dalian, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Genet Mol Res. 2014 Jan 10;13(1):134-8. doi: 10.4238/2014.January.10.4.
A (GT/CA)13-microsatellite-enriched genomic library of the false kelpfish Sebastiscus marmoratus was constructed, and 20 polymorphic microsatellite loci were isolated and characterized. The polymorphisms were investigated in 48 wild individuals from a single population collected from the northern Yellow Sea. The numbers of alleles per locus varied from 4-22 with an average of 9. The observed and expected heterozygosities of each locus ranged from 0.196-0.958 and from 0.487-0.942, with an average of 0.693 and 0.765, respectively. One locus significantly deviated from Hardy-Weinberg equilibrium, and one pair of loci was in linkage disequilibrium determined by Bonferroni's correction. Cross-species amplification was also conducted in the related species Inimicus japonicus, collected from East China Sea. The result showed that six loci could be amplified from I. japonicus DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of scorpionfish.
构建了褐菖鲉（Sebastiscus marmoratus）的一个富含(GT/CA)13微卫星的基因组文库，分离并鉴定了20个多态微卫星位点。在从黄海北部采集的单个种群的48个野生个体中研究了这些多态性。每个位点的等位基因数从4到22不等，平均为9个。每个位点的观察杂合度和期望杂合度分别在0.196至0.958和0.487至0.942之间，平均分别为0.693和0.765。一个位点显著偏离哈迪-温伯格平衡，通过邦费罗尼校正确定有一对位点处于连锁不平衡状态。还对从东海采集的相关物种日本鬼鲉（Inimicus japonicus）进行了跨物种扩增。结果表明，从日本鬼鲉的DNA中可以扩增出6个位点。这些多态性标记将有助于评估鲉科鱼类的遗传变异和种群结构。
