Amorim J A E, Mata L R, Lédo A S, Azevedo V C R, Silva A V C
Programa de Pós-Graduação em Biotecnologia em Recursos Naturais, Universidade Federal de Sergipe, Aracaju, SE, Brasil.
Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
Genet Mol Res. 2015 Feb 2;14(1):823-33. doi: 10.4238/2015.February.2.7.
In this study, we analyzed the genetic diversity and structure of remnants of mangaba populations in states of northeastern Brazil by applying 9 microsatellite markers previously developed to establish conservation strategies for germplasm and species preservation. Six to 20 individuals per population were analyzed, with a total of 94 individuals and 6 populations from the states of Ceará, Pernambuco, and Sergipe, Brazil. The intra-population positive fixation index (f) in all populations indicated inbreeding resulting from the lack of random mating. The mean genetic diversity index values GST, FST, and RST estimated for divergence among the 6 populations were 0.14 (P < 0.05), revealing moderate genetic differentiation. The smallest FST value (P ≥ 0.05) was observed between the Jacarecoara and Tapera populations (0.005) and the highest between the Barra dos Coqueiros and Jacarecoara populations (0.287). The Jacarecoara population was the most divergent among the populations analyzed. According to analysis of molecular variance results, the largest variation percentage resulted from variability within populations (83.18%). Bayesian clustering analysis showed the formation of 2 sets (K = 2). Our results are important for developing strategies for in situ conservation of the species, seed collection, and ex situ conservation. For both methods, conservation of the greatest possible genetic variability of the species is essential.
在本研究中，我们应用先前开发的9个微卫星标记，分析了巴西东北部各州曼加巴种群残余的遗传多样性和结构，以制定种质保护策略和物种保护计划。每个种群分析6至20个个体，共分析了来自巴西塞阿拉州、伯南布哥州和塞尔希培州的94个个体及6个种群。所有种群的种群内正向固定指数（f）表明，由于缺乏随机交配导致了近亲繁殖。为6个种群间的分化估算的平均遗传多样性指数GST、FST和RST值为0.14（P < 0.05），表明存在中等程度的遗传分化。在雅卡雷科阿拉种群和塔佩拉种群之间观察到最小的FST值（P ≥ 0.05）（0.005），在科凯罗斯角种群和雅卡雷科阿拉种群之间观察到最高的FST值（0.287）。在分析的种群中，雅卡雷科阿拉种群差异最大。根据分子方差分析结果，最大的变异百分比来自种群内变异（83.18%）。贝叶斯聚类分析显示形成了2组（K = 2）。我们的结果对于制定该物种的原地保护、种子采集和迁地保护策略很重要。对于这两种方法而言，保护该物种尽可能大的遗传变异性至关重要。
