Sultan Muhammad Tauseef, Butt Masood Sadiq, Karim Roselina, Ahmad Nisar, Ahmad Rabia Shabbir, Ahmad Waqas
Faculty of Food Science & Technology, University of Putra Malaysia, Serdang, Selangor, Malaysia.
National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
Pak J Pharm Sci. 2015 Mar;28(2):589-95.
The onset of 21st century witnessed the awareness among the masses regarding the diet-health linkages. The researchers attempted to explore traditional products/plants were in the domain of pharmacy and nutrition focussing on their health benefits. In the present research intervention, we investigate the role of Nigella sativa fixed oil (NSFO) and essential oil (NSEO) in improving antioxidant status and modulation of enzymes. The National Institute of Health (NIH) provided us 30 Sprague Dawley rats that were equally placed in three groups. The groups were fed on their respective diets (56 days) two experimental diets i.e. D2 (NSFO @ 4.0%) and D3 (NSEO @ 0.30%) and control. The indices pertaining to antioxidant status, antioxidant enzymes, and parameters pertaining to immunity were evaluated at 4 weeks interval. The experimental diets (NSFO@ 4.0% & NSEO@ 0.30%) modulated the activities of antioxidant enzymes i.e., catalase (CAT), superoxide dismutase (SOD), glutathione transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx), positively. Indices of antioxidant status like tocopherols and glutathione were in linear relationship with that of GPx, GR and GST (P<0.01). Myeloperoxidase activities were in negative correlation with GST (P<0.01) but positive correlation with some other parameters. In the nutshell, the fixed and essential oil of Nigella sativa are effective in improving the indices pertaining to antioxidant status, however, the immune boosting potential needs further clarification. However, authors are of the view that there is need to explore the molecular targets of Nigella sativa fixed and essential oils. Findings from such studies would be useful to validate this instant study for health promoting potential against diabetes mellitus and cardiovascular disorders.
21世纪伊始，大众开始意识到饮食与健康之间的联系。研究人员试图探索传统产品/植物在药学和营养领域的应用，重点关注它们对健康的益处。在本研究干预中，我们调查了黑种草籽油（NSFO）和精油（NSEO）在改善抗氧化状态和调节酶方面的作用。美国国立卫生研究院（NIH）为我们提供了30只斯普拉格-道利大鼠，并将它们平均分为三组。这些组分别喂食各自的饮食（56天），两种实验饮食即D2（4.0%的NSFO）和D3（0.30%的NSEO）以及对照组。每隔4周评估与抗氧化状态、抗氧化酶相关的指标以及与免疫相关的参数。实验饮食（4.0%的NSFO和0.30%的NSEO）对过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽转移酶（GST）、谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GPx）等抗氧化酶的活性有积极的调节作用。抗氧化状态指标如生育酚和谷胱甘肽与GPx、GR和GST呈线性关系（P<0.01）。髓过氧化物酶活性与GST呈负相关（P<0.01），但与其他一些参数呈正相关。简而言之，黑种草的固定油和精油在改善抗氧化状态指标方面是有效的，然而，其免疫增强潜力需要进一步阐明。不过，作者认为有必要探索黑种草固定油和精油的分子靶点。此类研究的结果将有助于验证本研究对糖尿病和心血管疾病的健康促进潜力。
