Du Bin, Han Shuping, Li Hongyan, Zhao Feifei, Su Xiangjie, Cao Xiaohui, Zhang Zhenzhong
School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, 450001, Zhengzhou, China.
Nanoscale. 2015 Mar 12;7(12):5411-26. doi: 10.1039/c4nr04257c.
Recently, nanoplatforms with multiple functions, such as tumor-targeting drug carriers, MRI, optical imaging, thermal therapy etc., have become popular in the field of cancer research. The present study reports a novel multi-functional liposome for cancer theranostics. A dual targeted drug delivery with radiofrequency-triggered drug release and imaging based on the magnetic field influence was used advantageously for tumor multi-mechanism therapy. In this system, the surface of fullerene (C60) was decorated with iron oxide nanoparticles, and PEGylation formed a hybrid nanosystem (C60-Fe3O4-PEG2000). Thermosensitive liposomes (dipalmitoylphosphatidylcholine, DPPC) with DSPE-PEG2000-folate wrapped up the hybrid nanosystem and docetaxel (DTX), which were designed to combine features of biological and physical (magnetic) drug targeting for fullerene radiofrequency-triggered drug release. The magnetic liposomes not only served as powerful tumor diagnostic magnetic resonance imaging (MRI) contrast agents, but also as powerful agents for photothermal ablation of tumors. Furthermore, a remarkable thermal therapy combined chemotherapy multi-functional liposome nanoplatform converted radiofrequency energy into thermal energy to release drugs from thermosensitive liposomes, which was also observed during both in vitro and in vivo treatment. The multi-functional liposomes also could selectively kill cancer cells in highly localized regions via their excellent active tumor targeting and magnetic targeted abilities.
近年来，具有多种功能的纳米平台，如肿瘤靶向药物载体、磁共振成像、光学成像、热疗等，在癌症研究领域变得很受欢迎。本研究报道了一种用于癌症诊疗的新型多功能脂质体。基于磁场影响的具有射频触发药物释放和成像功能的双靶向药物递送被有利地用于肿瘤多机制治疗。在该系统中，富勒烯（C60）表面用氧化铁纳米颗粒进行修饰，聚乙二醇化形成了一种混合纳米系统（C60-Fe3O4-PEG2000）。包裹有DSPE-PEG2000-叶酸的热敏脂质体（二棕榈酰磷脂酰胆碱，DPPC）包裹了混合纳米系统和多西他赛（DTX），其设计目的是结合生物和物理（磁性）药物靶向的特点，实现富勒烯射频触发的药物释放。磁性脂质体不仅作为强大的肿瘤诊断磁共振成像（MRI）造影剂，还作为强大的肿瘤光热消融剂。此外，一种显著的热疗联合化疗多功能脂质体纳米平台将射频能量转化为热能，以从热敏脂质体中释放药物，这在体外和体内治疗过程中均有观察到。多功能脂质体还可通过其出色的主动肿瘤靶向和磁性靶向能力，在高度局部化区域选择性杀死癌细胞。
