Tepler I, Morton C C, Shimizu A, Holcombe R F, Eddy R, Shows T B, Leder P
Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston MA 02115.
Am J Hum Genet. 1989 Nov;45(5):761-5.
We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.
我们使用了针对人IgE受体α链的cDNA探针来确定该人类基因的染色体定位。通过对体细胞杂交DNA面板进行Southern印迹分析以及染色体原位杂交相结合的方法，已将该基因定位到1号染色体长臂的q21 - q23区域。利用此cDNA探针，我们还鉴定出一种以孟德尔方式遗传的RsaI限制性片段长度多态性（RFLP）。1号染色体上的这种多态性标记对于旨在鉴定涉及IgE受体α链的疾病以及影响过敏反应的遗传因素的研究应具有重要价值。
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