Shows T, Eddy R, Haley L, Byers M, Henry M, Fujita T, Matsui H, Taniguchi T
Somat Cell Mol Genet. 1984 May;10(3):315-8. doi: 10.1007/BF01535253.
The human gene for interleukin 2 (IL2) was assigned to chromosome 4 using human-mouse somatic cell hybrids and Southern filter hybridization of cell hybrid DNA. To identify IL2, a recombinant DNA probe ( pIL2 - 50A ) was used which contained a human interleukin 2 cDNA insert which hybridized to a 3.5-kb fragment in human DNA when cleaved with the restriction enzyme EcoRI.
利用人-鼠体细胞杂种以及细胞杂种DNA的Southern印迹杂交技术，将人类白细胞介素2（IL2）基因定位于4号染色体。为了鉴定IL2，使用了一种重组DNA探针（pIL2 - 50A），该探针包含一个人类白细胞介素2 cDNA插入片段，用限制性内切酶EcoRI切割后，它能与人类DNA中的一个3.5kb片段杂交。
