在单细胞水平评估酵母丝状生长。
Evaluating yeast filamentous growth at the single-cell level.
作者信息
Cullen Paul J
机构信息
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260.
出版信息
Cold Spring Harb Protoc. 2015 Mar 2;2015(3):272-5. doi: 10.1101/pdb.prot085084.
Budding yeast can undergo filamentous growth in response to nutrient limitation. Filament formation can be examined under glucose-limiting conditions by the single-cell invasive growth assay, or under nitrogen-limiting conditions with the pseudohyphal growth assay, both described here. The single-cell assay allows robust quantitation of changes in budding pattern and cell length, and most cells in the population show the response. The pseudohyphal growth assay reveals filamentous patterns in larger microcolonies and adjoining subpopulations of cells. Historically, the single-cell assay has been used to study filamentous growth in haploid cells and the pseudohyphal growth assay in diploid cells. However, both assays can be used in either cell type.
出芽酵母在营养限制的情况下能够进行丝状生长。丝状生长可通过单细胞侵入性生长试验在葡萄糖限制条件下进行检测，或者通过假菌丝生长试验在氮限制条件下进行检测，这两种检测方法均在此处介绍。单细胞试验能够对芽殖模式和细胞长度的变化进行可靠的定量分析，并且群体中的大多数细胞都会表现出这种反应。假菌丝生长试验则揭示了较大微菌落以及相邻细胞亚群中的丝状模式。从历史上看，单细胞试验一直用于研究单倍体细胞中的丝状生长，而假菌丝生长试验用于研究二倍体细胞中的丝状生长。然而，这两种试验均可用于任何一种细胞类型。
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