Sang Yi, Cheng Chun, Tang Xiao-Feng, Zhang Mei-Fang, Lv Xiao-Bin
Department of Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, China E-mail :
Asian Pac J Cancer Prev. 2015;16(3):1197-200. doi: 10.7314/apjcp.2015.16.3.1197.
Breast cancer metastasis is a major cause of cancer-related death in women. However, markers for diagnosis of breast cancer metastasis are rare. Here, we reported that TET1, a tumor suppressor gene, was downregulated and hypermethylated in highly metastatic breast cancer cell lines. Moreover, silencing of TET1 in breast cancer cells increased the migration and spreading of breast cancer cells. In breast cancer clinical samples, TET1 expression was reduced in LN metastases compared with primary tissues. Besides, the methylation level of the TET1 promoter was increased significantly in LN metastases. Taken together, these findings indicate that promoter hypermethylation may contribute to the downregulation of TET1 and could be used as a promising marker for diagnosis in patients with breast cancer metastasis.
乳腺癌转移是女性癌症相关死亡的主要原因。然而，用于诊断乳腺癌转移的标志物很少。在此，我们报告肿瘤抑制基因TET1在高转移性乳腺癌细胞系中表达下调且发生高甲基化。此外，在乳腺癌细胞中沉默TET1会增加乳腺癌细胞的迁移和扩散。在乳腺癌临床样本中，与原发组织相比，TET1在淋巴结转移灶中的表达降低。此外，TET1启动子的甲基化水平在淋巴结转移灶中显著升高。综上所述，这些发现表明启动子高甲基化可能导致TET1下调，并有望作为乳腺癌转移患者诊断的标志物。
