Kang Seong-Il, Shin Hye-Sun, Kim Se-Jae
Department of Biology, Jeju National University.
Biol Pharm Bull. 2015;38(4):552-8. doi: 10.1248/bpb.b14-00700. Epub 2015 Jan 24.
Sinensetin is a rare polymethoxylated flavone (PMF) found in certain citrus fruits. In this study, we investigated the effects of sinensetin on lipid metabolism in 3T3-L1 cells. Sinensetin promoted adipogenesis in 3T3-L1 preadipocytes growing in incomplete differentiation medium, which did not contain 3-isobutyl-1-methylxanthine. Sinensetin up-regulated expression of the adipogenic transcription factors peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein (C/EBP) α, and sterol regulatory element-binding protein 1c. It also potentiated expression of C/EBPβ and activation of cAMP-responsive element-binding protein. Sinensetin enhanced activation of protein kinase A and increased intracellular cAMP levels in 3T3-L1 preadipocytes. In mature 3T3-L1 adipocytes, sinensetin stimulated lipolysis via a cAMP pathway. Taken together, these results suggest that sinensetin enhances adipogenesis and lipolysis by increasing cAMP levels in adipocytes.
橙皮素是一种在某些柑橘类水果中发现的罕见多甲氧基黄酮（PMF）。在本研究中，我们研究了橙皮素对3T3-L1细胞脂质代谢的影响。橙皮素促进了在不含3-异丁基-1-甲基黄嘌呤的不完全分化培养基中生长的3T3-L1前脂肪细胞的脂肪生成。橙皮素上调了脂肪生成转录因子过氧化物酶体增殖物激活受体γ、CCAAT/增强子结合蛋白（C/EBP）α和固醇调节元件结合蛋白1c的表达。它还增强了C/EBPβ的表达和cAMP反应元件结合蛋白的激活。橙皮素增强了蛋白激酶A的激活，并增加了3T3-L1前脂肪细胞内的cAMP水平。在成熟的3T3-L1脂肪细胞中，橙皮素通过cAMP途径刺激脂肪分解。综上所述，这些结果表明橙皮素通过增加脂肪细胞中的cAMP水平来增强脂肪生成和脂肪分解。
